Misregulated progesterone secretion and impaired pregnancy in Cyp11a1 transgenic mice

Yu Chien, Wei Cheng Cheng, Menq Rong Wu, Si Tse Jiang, Che Kun James Shen, Bon Chu Chung

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Normal pregnancy is supported by increased levels of progesterone (P4), which is secreted from ovarian luteal cells via enzymatic steps catalyzed by P450scc (CYP11A1) and HSD3B. The development and maintenance of corpora lutea during pregnancy, however, are less well understood. Here we used Cyp11a1 transgenic mice to delineate the steps of luteal cell differentiation during pregnancy. Cyp11a1 in a bacterial artificial chromosome was injected into mouse embryos to generate transgenic mice with transgene expression that recapitulated endogenous Cyp11a1 expression. Cyp11a1 transgenic females displayed reduced pregnancy rate, impaired implantation and placentation, and decreased litter size in utero, although they produced comparable numbers of blastocysts. The differentiation of transgenic luteal cells was delayed during early pregnancy as shown by the delayed activation of genes involved in steroidogenesis and cholesterol availability. Luteal cell mitochondria were elongated, and their numbers were reduced, with morphology and numbers similar to those observed in granulosa cells. Transgenic luteal cells accumulated lipid droplets and secreted less progesterone during early pregnancy. The progesterone level returned to normal on Gestation Day 9 but was not properly withdrawn at term, leading to delayed stillbirth. P4 supplementation rescued the implantation rates but not the ovarian defects. Thus, overexpression of Cyp11a1 disrupts normal development of the corpus luteum, leading to progesterone insufficiency during early pregnancy. Misregulation of the progesterone production in Cyp11a1 transgenic mice during pregnancy resulted in aberrant implantation, anomalous placentation, and delayed parturition.

Original languageEnglish
Article numberArticle 91
JournalBiology of Reproduction
Volume89
Issue number4
DOIs
Publication statusPublished - Jan 1 2013

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Transgenic Mice
Progesterone
Luteal Cells
Pregnancy
Placentation
Corpus Luteum Maintenance
Cholesterol Side-Chain Cleavage Enzyme
Bacterial Artificial Chromosomes
Litter Size
Stillbirth
Granulosa Cells
Corpus Luteum
Blastocyst
Pregnancy Rate
Transgenes
Transcriptional Activation
Cell Differentiation
Mitochondria
Embryonic Structures
Cholesterol

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Chien, Y., Cheng, W. C., Wu, M. R., Jiang, S. T., Shen, C. K. J., & Chung, B. C. (2013). Misregulated progesterone secretion and impaired pregnancy in Cyp11a1 transgenic mice. Biology of Reproduction, 89(4), [Article 91]. https://doi.org/10.1095/biolreprod.113.110833

Misregulated progesterone secretion and impaired pregnancy in Cyp11a1 transgenic mice. / Chien, Yu; Cheng, Wei Cheng; Wu, Menq Rong; Jiang, Si Tse; Shen, Che Kun James; Chung, Bon Chu.

In: Biology of Reproduction, Vol. 89, No. 4, Article 91, 01.01.2013.

Research output: Contribution to journalArticle

Chien, Y, Cheng, WC, Wu, MR, Jiang, ST, Shen, CKJ & Chung, BC 2013, 'Misregulated progesterone secretion and impaired pregnancy in Cyp11a1 transgenic mice', Biology of Reproduction, vol. 89, no. 4, Article 91. https://doi.org/10.1095/biolreprod.113.110833
Chien, Yu ; Cheng, Wei Cheng ; Wu, Menq Rong ; Jiang, Si Tse ; Shen, Che Kun James ; Chung, Bon Chu. / Misregulated progesterone secretion and impaired pregnancy in Cyp11a1 transgenic mice. In: Biology of Reproduction. 2013 ; Vol. 89, No. 4.
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