TY - JOUR
T1 - Mitochondria regulate the differentiation of stem cells from human exfoliated deciduous teeth
AU - Kato, Hiroki
AU - Pham, Thanh Thi Mai
AU - Yamaza, Haruyoshi
AU - Masuda, Keiji
AU - Hirofuji, Yuta
AU - Han, Xu
AU - Sato, Hiroshi
AU - Taguchi, Tomoaki
AU - Nonaka, Kazuaki
N1 - Funding Information:
Acknowledgments. We thank Drs. Y. Ninomiya, H. Nakanishi, T. Yamaza, and K. Sanematsu and all members of the Pediatric & Special Needs Dentistry at Kyushu University Hospital for the valuable suggestions, technical support, and materials. We appreciate the technical assistance provided by the Research Support Center, Research Center for Human Disease Modeling, Kyushu University Graduate School of Medical Sciences. This work was supported by JSPS KAKENHI (grant numbers 25670877 and 16K15839). The authors deny any conflicts of interest related to this study.
Publisher Copyright:
© 2017 The Author(s).
PY - 2017
Y1 - 2017
N2 - Stem cells from human exfoliated deciduous teeth (SHED) are isolated from the dental pulp tissue of primary teeth and can differentiate into neuronal cells. Although SHED are a desirable type of stem cells for transplantation therapy and for the study of neurological diseases, a large part of the neuronal differentiation machinery of SHED remains unclear. Recent studies have suggested that mitochondrial activity is involved in the differentiation of stem cells. In the present work, we investigated the neuronal differentiation machinery of SHED by focusing on mitochondrial activity. During neuronal differentiation of SHED, we observed increased mitochondrial membrane potential, increased mitochondrial DNA, and elongated mitochondria. Furthermore, to examine the demand for mitochondrial activity in neuronal differentiation, we then differentiated SHED into neuronal cells in the presence of rotenone, an inhibitor of mitochondrial respiratory chain complex I, and carbonyl cyanide m-chlorophenyl hydrazone (CCCP), a mitochondrial uncoupler, and found that neuronal differentiation was inhibited by treatment with rotenone and CCCP. These results indicated that increased mitochondrial activity was crucial for the neuronal differentiation of SHED.
AB - Stem cells from human exfoliated deciduous teeth (SHED) are isolated from the dental pulp tissue of primary teeth and can differentiate into neuronal cells. Although SHED are a desirable type of stem cells for transplantation therapy and for the study of neurological diseases, a large part of the neuronal differentiation machinery of SHED remains unclear. Recent studies have suggested that mitochondrial activity is involved in the differentiation of stem cells. In the present work, we investigated the neuronal differentiation machinery of SHED by focusing on mitochondrial activity. During neuronal differentiation of SHED, we observed increased mitochondrial membrane potential, increased mitochondrial DNA, and elongated mitochondria. Furthermore, to examine the demand for mitochondrial activity in neuronal differentiation, we then differentiated SHED into neuronal cells in the presence of rotenone, an inhibitor of mitochondrial respiratory chain complex I, and carbonyl cyanide m-chlorophenyl hydrazone (CCCP), a mitochondrial uncoupler, and found that neuronal differentiation was inhibited by treatment with rotenone and CCCP. These results indicated that increased mitochondrial activity was crucial for the neuronal differentiation of SHED.
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U2 - 10.1247/csf.17012
DO - 10.1247/csf.17012
M3 - Article
C2 - 28701634
AN - SCOPUS:85028636273
SN - 0386-7196
VL - 42
SP - 105
EP - 116
JO - Cell Structure and Function
JF - Cell Structure and Function
IS - 2
ER -
