Mitochondrial ABC transporter Atm1p is required for protection against oxidative stress and vacuolar functions in Schizosaccharomyces pombe

Tomoko Iwaki, Yasuko Fujita, Naotaka Tanaka, Yuko Giga-Hama, Kaoru Takegawa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A potential correlation between mitochondrial and vacuolar functions is known to exit in yeast. Fission yeast atm1+, SPAC15A10.01, encodes a putative half-type ABC transporter with an N-terminal mitochondrial-targeting signal. In an attempt to evaluate the possible involvement of mitochondrion in vacuole function, a functional analysis of atm1+ was performed by gene disruption. Growth of the atm1 mutant was inhibited in the presence of oxidizing agents, and S. cerevisiae Atm1p was found to complement this growth defect. atm1Δ cells exhibited defects in fluid-phase endocytosis and vacuolar fusion under hypotonic stress. GFP-tagged Atm1p was observed to be localized in the mitochondria. These data strongly suggest that fission yeast Atm1p was not only involved in protection against oxidative stress, but also played a role in vacuolar functions.

Original languageEnglish
Pages (from-to)2109-2116
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume69
Issue number11
DOIs
Publication statusPublished - Dec 8 2005
Externally publishedYes

Fingerprint

ABC transporters
Oxidative stress
ATP-Binding Cassette Transporters
Schizosaccharomyces
Schizosaccharomyces pombe
Yeast
Mitochondria
Oxidative Stress
mitochondria
oxidative stress
Osmotic Pressure
endocytosis
gene targeting
Growth
Endocytosis
Vacuoles
Oxidants
oxidants
vacuoles
Saccharomyces cerevisiae

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Food Science

Cite this

Mitochondrial ABC transporter Atm1p is required for protection against oxidative stress and vacuolar functions in Schizosaccharomyces pombe. / Iwaki, Tomoko; Fujita, Yasuko; Tanaka, Naotaka; Giga-Hama, Yuko; Takegawa, Kaoru.

In: Bioscience, Biotechnology and Biochemistry, Vol. 69, No. 11, 08.12.2005, p. 2109-2116.

Research output: Contribution to journalArticle

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