Mitochondrial division occurs concurrently with autophagosome formation but independently of Drp1 during mitophagy

Shun Ichi Yamashita, Xiulian Jin, Kentaro Furukawa, Maho Hamasaki, Akiko Nezu, Hidenori Otera, Tetsu Saigusa, Tamotsu Yoshimori, Yasuyoshi Sakai, Katsuyoshi Mihara, Tomotake Kanki

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Mitophagy is thought to play an important role in mitochondrial quality control. Mitochondrial division is believed to occur first, and autophagosome formation subsequently occurs to enwrap mitochondria as a process of mitophagy. However, there has not been any temporal analysis of mitochondrial division and autophagosome formation in mitophagy. Therefore, the relationships among these processes remain unclear. We show that the mitochondrial division factor Dnm1 in yeast or Drp1 in mammalian cells is dispensable for mitophagy. Autophagosome formation factors, such as FIP200, ATG14, and WIPIs, were essential for the mitochondrial division for mitophagy. Live-cell imaging showed that isolation membranes formed on the mitochondria. A small portion of the mitochondria then divided from parental mitochondria simultaneously with the extension of isolation membranes and autophagosome formation. These findings suggest the presence of a mitophagy process in which mitochondrial division for mitophagy is accomplished together with autophagosome formation.

Original languageEnglish
Pages (from-to)649-665
Number of pages17
JournalJournal of Cell Biology
Volume215
Issue number5
DOIs
Publication statusPublished - Jan 1 2016

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Mitochondrial Degradation
Mitochondria
Membranes
Autophagosomes
Quality Control
Yeasts

All Science Journal Classification (ASJC) codes

  • Cell Biology

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Mitochondrial division occurs concurrently with autophagosome formation but independently of Drp1 during mitophagy. / Yamashita, Shun Ichi; Jin, Xiulian; Furukawa, Kentaro; Hamasaki, Maho; Nezu, Akiko; Otera, Hidenori; Saigusa, Tetsu; Yoshimori, Tamotsu; Sakai, Yasuyoshi; Mihara, Katsuyoshi; Kanki, Tomotake.

In: Journal of Cell Biology, Vol. 215, No. 5, 01.01.2016, p. 649-665.

Research output: Contribution to journalArticle

Yamashita, SI, Jin, X, Furukawa, K, Hamasaki, M, Nezu, A, Otera, H, Saigusa, T, Yoshimori, T, Sakai, Y, Mihara, K & Kanki, T 2016, 'Mitochondrial division occurs concurrently with autophagosome formation but independently of Drp1 during mitophagy', Journal of Cell Biology, vol. 215, no. 5, pp. 649-665. https://doi.org/10.1083/jcb.201605093
Yamashita, Shun Ichi ; Jin, Xiulian ; Furukawa, Kentaro ; Hamasaki, Maho ; Nezu, Akiko ; Otera, Hidenori ; Saigusa, Tetsu ; Yoshimori, Tamotsu ; Sakai, Yasuyoshi ; Mihara, Katsuyoshi ; Kanki, Tomotake. / Mitochondrial division occurs concurrently with autophagosome formation but independently of Drp1 during mitophagy. In: Journal of Cell Biology. 2016 ; Vol. 215, No. 5. pp. 649-665.
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AU - Otera, Hidenori

AU - Saigusa, Tetsu

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