Mitochondrial DNA-like sequences in the human nuclear genome. Characterization and implications in the evolution of mitochondrial DNA

Makoto Fukuda, Shoji Wakasugi, Teruhisa Tsuzuki, Hisayuki Nomiyama, Kazunori Shimada, Takashi Miyata

Research output: Contribution to journalArticle

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Abstract

Thirty-three phage clones carrying DNAs homologous to human mitochondrial DNA (mtDNA) were isolated from two independently constructed human gene libraries, and the region and extent of homology of mtDNA-like sequences carried by these clones were examined in hybridization experiments. Each phage clone contained DNA sequences homologous to various parts of the mtDNA and the extent of homology differed from clone to clone. From the efficiency of the library screening, it was estimated that human nuclear DNA contains at least several hundred copies of mtDNA-like fragments. Four clones carrying nuclear DNA sequences homologous to the mitochondrial Unidentified Reading Frame (URF) 4 and URF5 regions were chosen for further studies, and their structures were analyzed by DNA sequencing. Comparison of these mtDNA-like sequences with that of mtDNAs of several mammalian species revealed conservation of a part of the structures present in direct ancestral mtDNAs. The mtDNA fragments seem to have been continuously integrated into mammalian nuclear DNA during evolution.

Original languageEnglish
Pages (from-to)257-266
Number of pages10
JournalJournal of Molecular Biology
Volume186
Issue number2
DOIs
Publication statusPublished - Nov 20 1985

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Human Genome
Mitochondrial DNA
Clone Cells
Bacteriophages
DNA
Gene Library
DNA Sequence Analysis
Open Reading Frames

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Cite this

Mitochondrial DNA-like sequences in the human nuclear genome. Characterization and implications in the evolution of mitochondrial DNA. / Fukuda, Makoto; Wakasugi, Shoji; Tsuzuki, Teruhisa; Nomiyama, Hisayuki; Shimada, Kazunori; Miyata, Takashi.

In: Journal of Molecular Biology, Vol. 186, No. 2, 20.11.1985, p. 257-266.

Research output: Contribution to journalArticle

Fukuda, Makoto ; Wakasugi, Shoji ; Tsuzuki, Teruhisa ; Nomiyama, Hisayuki ; Shimada, Kazunori ; Miyata, Takashi. / Mitochondrial DNA-like sequences in the human nuclear genome. Characterization and implications in the evolution of mitochondrial DNA. In: Journal of Molecular Biology. 1985 ; Vol. 186, No. 2. pp. 257-266.
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