Abstract
Mitochondrial pyruvate carrier (MPC) is known to cause different expressions in normal and cancer cells. We observed a change in phenotype with the suppression of MPC expression. We knocked down MPC1 and/or MPC2 using siRNA or shRNA. We observed its cell morphology and accompanying molecular marker. Furthermore, the radioresistance of the MPC knockdown cell line was examined using a colony formation assay. MPC1-suppressed cells changed their morphology to a spindle shape. Epithelial-mesenchymal transition (EMT) was suspected, and examination of the EMT marker by PCR showed a decrease in E-cadherin and an increase in fibronectin. Focusing on glutamine metabolism as the mechanism of this phenomenon, we knocked down the glutamine-metabolizing enzyme glutaminase (GLS). EMT was also observed in GLS-suppressed cells. Furthermore, when MPC1-suppressed cells were cultured in a glutamine-deficient medium, changes in EMT markers were suppressed. In addition, MPC1-suppressed cells also increased with a significant difference in radioresistance. Decreased MPC1 expression favorably affects EMT and radioresistance of cancer.
| Original language | English |
|---|---|
| Pages (from-to) | 1331-1339 |
| Number of pages | 9 |
| Journal | Cancer Science |
| Volume | 110 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Apr 2019 |
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All Science Journal Classification (ASJC) codes
- Oncology
- Cancer Research
Cite this
Mitochondrial pyruvate carrier 1 expression controls cancer epithelial-mesenchymal transition and radioresistance. / Takaoka, Yuji; Konno, Masamitsu; Koseki, Jun; Colvin, Hugh; Asai, Ayumu; Tamari, Keisuke; Satoh, Taroh; Mori, Masaki; Doki, Yuichiro; Ogawa, Kazuhiko; Ishii, Hideshi.
In: Cancer Science, Vol. 110, No. 4, 04.2019, p. 1331-1339.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mitochondrial pyruvate carrier 1 expression controls cancer epithelial-mesenchymal transition and radioresistance
AU - Takaoka, Yuji
AU - Konno, Masamitsu
AU - Koseki, Jun
AU - Colvin, Hugh
AU - Asai, Ayumu
AU - Tamari, Keisuke
AU - Satoh, Taroh
AU - Mori, Masaki
AU - Doki, Yuichiro
AU - Ogawa, Kazuhiko
AU - Ishii, Hideshi
PY - 2019/4
Y1 - 2019/4
N2 - Mitochondrial pyruvate carrier (MPC) is known to cause different expressions in normal and cancer cells. We observed a change in phenotype with the suppression of MPC expression. We knocked down MPC1 and/or MPC2 using siRNA or shRNA. We observed its cell morphology and accompanying molecular marker. Furthermore, the radioresistance of the MPC knockdown cell line was examined using a colony formation assay. MPC1-suppressed cells changed their morphology to a spindle shape. Epithelial-mesenchymal transition (EMT) was suspected, and examination of the EMT marker by PCR showed a decrease in E-cadherin and an increase in fibronectin. Focusing on glutamine metabolism as the mechanism of this phenomenon, we knocked down the glutamine-metabolizing enzyme glutaminase (GLS). EMT was also observed in GLS-suppressed cells. Furthermore, when MPC1-suppressed cells were cultured in a glutamine-deficient medium, changes in EMT markers were suppressed. In addition, MPC1-suppressed cells also increased with a significant difference in radioresistance. Decreased MPC1 expression favorably affects EMT and radioresistance of cancer.
AB - Mitochondrial pyruvate carrier (MPC) is known to cause different expressions in normal and cancer cells. We observed a change in phenotype with the suppression of MPC expression. We knocked down MPC1 and/or MPC2 using siRNA or shRNA. We observed its cell morphology and accompanying molecular marker. Furthermore, the radioresistance of the MPC knockdown cell line was examined using a colony formation assay. MPC1-suppressed cells changed their morphology to a spindle shape. Epithelial-mesenchymal transition (EMT) was suspected, and examination of the EMT marker by PCR showed a decrease in E-cadherin and an increase in fibronectin. Focusing on glutamine metabolism as the mechanism of this phenomenon, we knocked down the glutamine-metabolizing enzyme glutaminase (GLS). EMT was also observed in GLS-suppressed cells. Furthermore, when MPC1-suppressed cells were cultured in a glutamine-deficient medium, changes in EMT markers were suppressed. In addition, MPC1-suppressed cells also increased with a significant difference in radioresistance. Decreased MPC1 expression favorably affects EMT and radioresistance of cancer.
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UR - http://www.scopus.com/inward/citedby.url?scp=85063898476&partnerID=8YFLogxK
U2 - 10.1111/cas.13980
DO - 10.1111/cas.13980
M3 - Article
C2 - 30801869
AN - SCOPUS:85063898476
VL - 110
SP - 1331
EP - 1339
JO - Cancer Science
JF - Cancer Science
SN - 1347-9032
IS - 4
ER -