Abstract
Correlation between the expression of the α-tropomyosin isoforms and cell growth was investigated in rat aortic smooth muscle cells. The levels of exon 1a, exons 1a + 2a (smooth muscle type) and exons 1a + 2b (non-smooth muscle type) were determined by reverse transcription-polymerase chain reaction (RT-PCR). When the cells were cultured, the level of exons 1a + 2b transiently increased while reaching a maximum at 3-5 days. When the serum- deprived confluent cells were stimulated with 3-20% serum for 1.5 h, the level of exons 1a + 2b increased by about twofold. The 1-(5- isoquinolinesulphonyl)-2-methylpiperazine (H-7) but not 2-[1-(3- dimethylaminopropyl)-1H-indol-3-yl]-3-(1H-indol-3-yl)-maleimide (GF 109203X) inhibited this up-regulation. Phorbol-12,13-dibutyrate (PDB) mimicked the effect of serum. The DNA synthesis as determined by the incorporation of 5- bromo-2'-deoxy-uridine (BrdU) was not enhanced by the 1.5 h stimulation with serum or phorbol ester. The up-regulation of non-smooth muscle isoform of α- tropomyosin occurred during G0/G1 transition before entering S phase. Protein phosphorylation is suggested to be involved in the up-regulation. However, the responsible kinase(s) remain to be elucidated. (C) 2000 Elsevier Science B.V.
| Original language | English |
|---|---|
| Pages (from-to) | 209-218 |
| Number of pages | 10 |
| Journal | European Journal of Pharmacology |
| Volume | 406 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Oct 13 2000 |
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All Science Journal Classification (ASJC) codes
- Pharmacology
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Mitogen-induced up-regulation of non-smooth muscle isoform of α- tropomyosin in rat aortic smooth muscle cells. / Hirano, Katsuya; Hirano, Mayumi; Eto, Wakako; Nishimura, Junji; Kanaide, Hideo.
In: European Journal of Pharmacology, Vol. 406, No. 2, 13.10.2000, p. 209-218.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mitogen-induced up-regulation of non-smooth muscle isoform of α- tropomyosin in rat aortic smooth muscle cells
AU - Hirano, Katsuya
AU - Hirano, Mayumi
AU - Eto, Wakako
AU - Nishimura, Junji
AU - Kanaide, Hideo
PY - 2000/10/13
Y1 - 2000/10/13
N2 - Correlation between the expression of the α-tropomyosin isoforms and cell growth was investigated in rat aortic smooth muscle cells. The levels of exon 1a, exons 1a + 2a (smooth muscle type) and exons 1a + 2b (non-smooth muscle type) were determined by reverse transcription-polymerase chain reaction (RT-PCR). When the cells were cultured, the level of exons 1a + 2b transiently increased while reaching a maximum at 3-5 days. When the serum- deprived confluent cells were stimulated with 3-20% serum for 1.5 h, the level of exons 1a + 2b increased by about twofold. The 1-(5- isoquinolinesulphonyl)-2-methylpiperazine (H-7) but not 2-[1-(3- dimethylaminopropyl)-1H-indol-3-yl]-3-(1H-indol-3-yl)-maleimide (GF 109203X) inhibited this up-regulation. Phorbol-12,13-dibutyrate (PDB) mimicked the effect of serum. The DNA synthesis as determined by the incorporation of 5- bromo-2'-deoxy-uridine (BrdU) was not enhanced by the 1.5 h stimulation with serum or phorbol ester. The up-regulation of non-smooth muscle isoform of α- tropomyosin occurred during G0/G1 transition before entering S phase. Protein phosphorylation is suggested to be involved in the up-regulation. However, the responsible kinase(s) remain to be elucidated. (C) 2000 Elsevier Science B.V.
AB - Correlation between the expression of the α-tropomyosin isoforms and cell growth was investigated in rat aortic smooth muscle cells. The levels of exon 1a, exons 1a + 2a (smooth muscle type) and exons 1a + 2b (non-smooth muscle type) were determined by reverse transcription-polymerase chain reaction (RT-PCR). When the cells were cultured, the level of exons 1a + 2b transiently increased while reaching a maximum at 3-5 days. When the serum- deprived confluent cells were stimulated with 3-20% serum for 1.5 h, the level of exons 1a + 2b increased by about twofold. The 1-(5- isoquinolinesulphonyl)-2-methylpiperazine (H-7) but not 2-[1-(3- dimethylaminopropyl)-1H-indol-3-yl]-3-(1H-indol-3-yl)-maleimide (GF 109203X) inhibited this up-regulation. Phorbol-12,13-dibutyrate (PDB) mimicked the effect of serum. The DNA synthesis as determined by the incorporation of 5- bromo-2'-deoxy-uridine (BrdU) was not enhanced by the 1.5 h stimulation with serum or phorbol ester. The up-regulation of non-smooth muscle isoform of α- tropomyosin occurred during G0/G1 transition before entering S phase. Protein phosphorylation is suggested to be involved in the up-regulation. However, the responsible kinase(s) remain to be elucidated. (C) 2000 Elsevier Science B.V.
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U2 - 10.1016/S0014-2999(00)00681-6
DO - 10.1016/S0014-2999(00)00681-6
M3 - Article
VL - 406
SP - 209
EP - 218
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 2
ER -