TY - JOUR
T1 - Mitogen-induced up-regulation of non-smooth muscle isoform of α- tropomyosin in rat aortic smooth muscle cells
AU - Hirano, Katsuya
AU - Hirano, Mayumi
AU - Eto, Wakako
AU - Nishimura, Junji
AU - Kanaide, Hideo
N1 - Funding Information:
We thank Mr. Brian Quinn for comments and help with the manuscript. This study was supported in part by Grants-in-Aid for Scientific Research (No. 10557072, 11838013, 11670687), for the Encouragement of Young Scientists (No. 10770308) from the Ministry of Education, Science, Sports and Culture, Japan, by the Research Grant for Cardiovascular Diseases (11C-1) from the Ministry of Health and Welfare, Japan, and by grants from the Vehicle Racing Commemorative Foundation, the Foundation for the Promotion of Clinical Medicine, the Suzuken Memorial Foundation and KANZAWA Medical Research Foundation.
PY - 2000/10/13
Y1 - 2000/10/13
N2 - Correlation between the expression of the α-tropomyosin isoforms and cell growth was investigated in rat aortic smooth muscle cells. The levels of exon 1a, exons 1a + 2a (smooth muscle type) and exons 1a + 2b (non-smooth muscle type) were determined by reverse transcription-polymerase chain reaction (RT-PCR). When the cells were cultured, the level of exons 1a + 2b transiently increased while reaching a maximum at 3-5 days. When the serum- deprived confluent cells were stimulated with 3-20% serum for 1.5 h, the level of exons 1a + 2b increased by about twofold. The 1-(5- isoquinolinesulphonyl)-2-methylpiperazine (H-7) but not 2-[1-(3- dimethylaminopropyl)-1H-indol-3-yl]-3-(1H-indol-3-yl)-maleimide (GF 109203X) inhibited this up-regulation. Phorbol-12,13-dibutyrate (PDB) mimicked the effect of serum. The DNA synthesis as determined by the incorporation of 5- bromo-2'-deoxy-uridine (BrdU) was not enhanced by the 1.5 h stimulation with serum or phorbol ester. The up-regulation of non-smooth muscle isoform of α- tropomyosin occurred during G0/G1 transition before entering S phase. Protein phosphorylation is suggested to be involved in the up-regulation. However, the responsible kinase(s) remain to be elucidated. (C) 2000 Elsevier Science B.V.
AB - Correlation between the expression of the α-tropomyosin isoforms and cell growth was investigated in rat aortic smooth muscle cells. The levels of exon 1a, exons 1a + 2a (smooth muscle type) and exons 1a + 2b (non-smooth muscle type) were determined by reverse transcription-polymerase chain reaction (RT-PCR). When the cells were cultured, the level of exons 1a + 2b transiently increased while reaching a maximum at 3-5 days. When the serum- deprived confluent cells were stimulated with 3-20% serum for 1.5 h, the level of exons 1a + 2b increased by about twofold. The 1-(5- isoquinolinesulphonyl)-2-methylpiperazine (H-7) but not 2-[1-(3- dimethylaminopropyl)-1H-indol-3-yl]-3-(1H-indol-3-yl)-maleimide (GF 109203X) inhibited this up-regulation. Phorbol-12,13-dibutyrate (PDB) mimicked the effect of serum. The DNA synthesis as determined by the incorporation of 5- bromo-2'-deoxy-uridine (BrdU) was not enhanced by the 1.5 h stimulation with serum or phorbol ester. The up-regulation of non-smooth muscle isoform of α- tropomyosin occurred during G0/G1 transition before entering S phase. Protein phosphorylation is suggested to be involved in the up-regulation. However, the responsible kinase(s) remain to be elucidated. (C) 2000 Elsevier Science B.V.
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U2 - 10.1016/S0014-2999(00)00681-6
DO - 10.1016/S0014-2999(00)00681-6
M3 - Article
C2 - 11020483
AN - SCOPUS:0034644872
VL - 406
SP - 209
EP - 218
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 2
ER -