Mitogenic and Chemotactic Responses of Human Periodontal Ligament Cells to the Different Isoforms of Platelet-derived Growth Factor

L. A. Boyan, G. Bhargava, F. Nishimura, R. Orman, R. Price, V. P. Terranova

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Abstract

A major focus of studies that center on regeneration of the periodontium is to determine the efficacy of the use of polypeptide growth factors. Platelet-derived growth factor has been reported to be a possible agent for clinical use. PDGF has various isoforms. Therefore, we decided to study the mitogenic and chemotactic responses of human periodontal ligament (PDL) cells to recombinant human PDGF-AB, AA, and BB. Addition of each isoform of PDGF to in vitro mitogenesis assays induced PDL cell proliferation in a dose-dependent manner. The maximum mitogenic effect was evident at the concentration of 100 ng/mL. In these assays, PDGF-BB was found to be the most potent mitogen. PDGF-AB elicited an intermediate response, and PDGF-AA was the least effective. The results of chemotaxis assays closely parallel those of the mitogenesis assays. PDGF-BB exhibited the most potent chemotactic effect. The maximal effect was observed at 10 ng/mL. The findings of these experiments indicate that PDGF-BB is more effective than the other isoforms in promoting mitogenesis and chemotaxis of PDL cells in vitro, and may therefore be a suitable ethical pharmaceutical for use in periodontal regeneration procedures.

Original languageEnglish
Pages (from-to)1593-1600
Number of pages8
JournalJournal of Dental Research
Volume73
Issue number10
DOIs
Publication statusPublished - Oct 1994
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Dentistry(all)

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