Mitogenic and Chemotactic Responses of Human Periodontal Ligament Cells to the Different Isoforms of Platelet-derived Growth Factor

L. A. Boyan, G. Bhargava, Fusanori Nishimura, R. Orman, R. Price, V. P. Terranova

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Abstract

A major focus of studies that center on regeneration of the periodontium is to determine the efficacy of the use of polypeptide growth factors. Platelet-derived growth factor has been reported to be a possible agent for clinical use. PDGF has various isoforms. Therefore, we decided to study the mitogenic and chemotactic responses of human periodontal ligament (PDL) cells to recombinant human PDGF-AB, AA, and BB. Addition of each isoform of PDGF to in vitro mitogenesis assays induced PDL cell proliferation in a dose-dependent manner. The maximum mitogenic effect was evident at the concentration of 100 ng/mL. In these assays, PDGF-BB was found to be the most potent mitogen. PDGF-AB elicited an intermediate response, and PDGF-AA was the least effective. The results of chemotaxis assays closely parallel those of the mitogenesis assays. PDGF-BB exhibited the most potent chemotactic effect. The maximal effect was observed at 10 ng/mL. The findings of these experiments indicate that PDGF-BB is more effective than the other isoforms in promoting mitogenesis and chemotaxis of PDL cells in vitro, and may therefore be a suitable ethical pharmaceutical for use in periodontal regeneration procedures.

Original languageEnglish
Pages (from-to)1593-1600
Number of pages8
JournalJournal of Dental Research
Volume73
Issue number10
DOIs
Publication statusPublished - Jan 1 1994

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Periodontal Ligament
Platelet-Derived Growth Factor
Protein Isoforms
Chemotaxis
Regeneration
Periodontium
Mitogens
Intercellular Signaling Peptides and Proteins
Cell Proliferation
Peptides
platelet-derived growth factor BB
Pharmaceutical Preparations
platelet-derived growth factor A
platelet-derived growth factor AB
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Mitogenic and Chemotactic Responses of Human Periodontal Ligament Cells to the Different Isoforms of Platelet-derived Growth Factor. / Boyan, L. A.; Bhargava, G.; Nishimura, Fusanori; Orman, R.; Price, R.; Terranova, V. P.

In: Journal of Dental Research, Vol. 73, No. 10, 01.01.1994, p. 1593-1600.

Research output: Contribution to journalArticle

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