Modeling analysis of inositol 1,4,5-trisphosphate receptor-mediated Ca2+ mobilization under the control of glucagon-like peptide-1 in mouse pancreatic β-cells

Yukari Takeda, Takao Shimayoshi, George G. Holz, Akinori Noma

Research output: Contribution to journalArticle

Abstract

Glucagon-like peptide-1 (GLP-1) is an intestinally derived blood glucose-lowering hormone that potentiates glucose-stimulated insulin secretion from pancreatic β-cells. The secretagogue action of GLP-1 is explained, at least in part, by its ability to stimulate cAMP production so that cAMP may facilitate the release of Ca(2+) from inositol trisphosphate receptor (IP3R)-regulated Ca(2+) stores. However, a quantitative model has yet to be provided that explains the molecular mechanisms and dynamic processes linking GLP-1-stimulated cAMP production to Ca(2+) mobilization. Here, we performed simulation studies to investigate how GLP-1 alters the abilities of Ca(2+) and IP3 to act as coagonists at IP3R Ca(2+) release channels. A new dynamic model was constructed based on the Kaftan model, which demonstrates dual steady-state allosteric regulation of the IP3R by Ca(2+) and IP3. Data obtained from β-cells were then analyzed to understand how GLP-1 facilitates IP3R-mediated Ca(2+) mobilization when UV flash photolysis is used to uncage Ca(2+) and IP3 intracellularly. When the dynamic model for IP3R activation was incorporated into a minimal cell model, the Ca(2+) transients and oscillations induced by GLP-1 were successfully reconstructed. Simulation studies indicated that transient and oscillatory responses to GLP-1 were produced by sequential positive and negative feedback regulation due to fast activation and slow inhibition of the IP3R by Ca(2+). The slow rate of Ca(2+)-dependent inhibition was revealed to provide a remarkable contribution to the time course of the decay of cytosolic Ca(2+) transients. It also served to drive and pace Ca(2+) oscillations that are significant when evaluating how GLP-1 stimulates insulin secretion.

Original languageEnglish
Pages (from-to)C337-C347
JournalAmerican journal of physiology. Cell physiology
Volume310
Issue number5
DOIs
Publication statusPublished - Mar 1 2016
Externally publishedYes

Fingerprint

Inositol 1,4,5-Trisphosphate Receptors
Glucagon-Like Peptide 1
Allosteric Regulation
Insulin
Photolysis
Inositol
Molecular Dynamics Simulation
Blood Glucose
Hormones
Glucose

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cell Biology

Cite this

Modeling analysis of inositol 1,4,5-trisphosphate receptor-mediated Ca2+ mobilization under the control of glucagon-like peptide-1 in mouse pancreatic β-cells. / Takeda, Yukari; Shimayoshi, Takao; Holz, George G.; Noma, Akinori.

In: American journal of physiology. Cell physiology, Vol. 310, No. 5, 01.03.2016, p. C337-C347.

Research output: Contribution to journalArticle

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