Modes of overinitiation, dnaA gene expression, and inhibition of cell division in a novel cold-sensitive hda mutant of Escherichia coli

Kazuyuki Fujimitsu, Masayuki Su'etsugu, Yoko Yamaguchi, Kensaku Mazda, Nisi Fu, Hironori Kawakami, Tsutomu Katayama

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The chromosomal replication cycle is strictly coordinated with cell cycle progression in Escherichia coli. ATP-DnaA initiates replication, leading to loading of the DNA polymerase III holoenzyme. The DNA-loaded form of the β clamp subunit of the polymerase binds the Hda protein, which promotes ATP-DnaA hydrolysis, yielding inactive ADP-DnaA. This regulation is required to repress overinitiation. In this study, we have isolated a novel cold-sensitive hda mutant, the hda-185 mutant. The hda-185 mutant caused overinitiation of chromosomal replication at 25°C, which most likely led to blockage of replication fork progress. Consistently, the inhibition of colony formation at 25°C was suppressed by disruption of the diaA gene, an initiation stimulator. Disruption of the seqA gene, an initiation inhibitor, showed synthetic lethality with hda-185 even at 42°C. The cellular ATP-DnaA level was increased in an hda-185-dependent manner. The cellular concentrations of DnaA protein and dnaA mRNA were comparable at 25°C to those in a wild-type hda strain. We also found that multiple copies of the ribonucleotide reductase genes (nrdAB or nrdEF) or dnaB gene repressed overinitiation. The cellular levels of dATP and dCTP were elevated in cells bearing multiple copies of nrdAB. The catalytic site within NrdA was required for multicopy suppression, suggesting the importance of an active form of NrdA or elevated levels of deoxyribonucleotides in inhibition of overinitiation in the hda-185 cells. Cell division in the hda-185 mutant was inhibited at 25°C in a LexA regulon-independent manner, suggesting that overinitiation in the hda-185 mutant induced a unique division inhibition pathway.

Original languageEnglish
Pages (from-to)5368-5381
Number of pages14
JournalJournal of bacteriology
Volume190
Issue number15
DOIs
Publication statusPublished - Aug 1 2008

Fingerprint

Cell Division
Escherichia coli
Gene Expression
Adenosine Triphosphate
Genes
Deoxyribonucleotides
DNA Polymerase III
Ribonucleotide Reductases
Regulon
Holoenzymes
Adenosine Diphosphate
Catalytic Domain
Cell Cycle
Proteins
Hydrolysis
Messenger RNA
DNA

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Modes of overinitiation, dnaA gene expression, and inhibition of cell division in a novel cold-sensitive hda mutant of Escherichia coli. / Fujimitsu, Kazuyuki; Su'etsugu, Masayuki; Yamaguchi, Yoko; Mazda, Kensaku; Fu, Nisi; Kawakami, Hironori; Katayama, Tsutomu.

In: Journal of bacteriology, Vol. 190, No. 15, 01.08.2008, p. 5368-5381.

Research output: Contribution to journalArticle

Fujimitsu, Kazuyuki ; Su'etsugu, Masayuki ; Yamaguchi, Yoko ; Mazda, Kensaku ; Fu, Nisi ; Kawakami, Hironori ; Katayama, Tsutomu. / Modes of overinitiation, dnaA gene expression, and inhibition of cell division in a novel cold-sensitive hda mutant of Escherichia coli. In: Journal of bacteriology. 2008 ; Vol. 190, No. 15. pp. 5368-5381.
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