Modification of fatty acid composition by over- and antisense-expression of a microsomal ω-3 fatty acid desaturase gene in transgenic tobacco

Tatsurou Hamada, Hiroaki Kodama, Mitsuo Nishimura, Koh Iba

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

ω-3 fatty acid desaturases, which catalyse the conversion of linoleic acid (18:2) to linolenic acid (18:3) in lipids, are located in the microsomes and plastid membranes. Transgenic tobacco plants were produced that express the transcripts of a tobacco microsomal ω-3 fatty acid desaturase gene (NtFad3) in antisense and sense orientations under the control of the cauliflower mosaic virus 35S promoter. The antisease construct has the 0.5-kb fragment of the NtFad3 cDNA containing a 3′-flanking region and a part of the coding region in antisense orientation. The antisense-transformant lines showed decreases of the steady-state NtFad3 mRNA level to 30% of the control plants. In these lines, the 18:3 content decreased to about 80% in root tissues and to about 70-80% in leaf tissues when compared with the control plants. The sense construct has the 1.4-kb full-length cDNA of NtFad3. In one of the sense-transformant lines, the NtFad3 mRNA level increased 8 times when compared with that of the control plants. In this line, the 18:3 content increased by about 1.5-fold in root tissues and by about 1.1-fold in leaf tissues. These results indicate that the up- and down-regulation of the transcript level in the microsomal ω-3 fatty acid desaturase gene is useful to modify the 18:3 content in the vegetative tissues of higher plants.

Original languageEnglish
Pages (from-to)115-121
Number of pages7
JournalTransgenic Research
Volume5
Issue number2
DOIs
Publication statusPublished - Jan 1 1996

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Fatty Acid Desaturases
stearoyl-CoA desaturase
Tobacco
tobacco
Fatty Acids
fatty acid composition
genetically modified organisms
Genes
genes
Complementary DNA
Caulimovirus
3' Flanking Region
Messenger RNA
Plastids
alpha-Linolenic Acid
Cauliflower mosaic virus
Genetically Modified Plants
microsomes
Linoleic Acid
Microsomes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Animal Science and Zoology
  • Genetics
  • Agronomy and Crop Science

Cite this

Modification of fatty acid composition by over- and antisense-expression of a microsomal ω-3 fatty acid desaturase gene in transgenic tobacco. / Hamada, Tatsurou; Kodama, Hiroaki; Nishimura, Mitsuo; Iba, Koh.

In: Transgenic Research, Vol. 5, No. 2, 01.01.1996, p. 115-121.

Research output: Contribution to journalArticle

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