Modification of gold nanorods using phosphatidylcholine to reduce cytotoxicity

Hironobu Takahashi; Yasuro Niidome; Takuro Niidome; Kenji Kaneko; Hideya Kawasaki; Sunao Yamada

doi:10.1021/la0520029

Modification of gold nanorods using phosphatidylcholine to reduce cytotoxicity

Hironobu Takahashi, Yasuro Niidome, Takuro Niidome, Kenji Kaneko, Hideya Kawasaki, Sunao Yamada

Research output: Contribution to journal › Article › peer-review

368 Citations (Scopus)

Abstract

Hexadecyltrimethylammonium bromide (CTAB), which is necessary for the preparation of gold nanorods (NRs), was extracted from a NR solution into a chloroform phase containing phosphatidylcholine (PC). After three extractions, the zeta potential of the NRs remained positive, but its magnitude decreased from+67 ±1 to+15± 1 mV. Transmission electron microscopy and energy-dispersive X-ray analysis indicated that the NRs were passivated with PC. The PC layer on the NR surface contributed to the prevention of NR aggregation. The PC-passivated NRs showed low cytotoxicity in comparison with twice-centrifuged NRs. It was shown that a negligible amount of CTAB was dispersed in the NR solution after the extraction. The extraction using a chloroform phase containing PC was found to be a convenient way of replacing the CTAB with alternative capping agents such as PC. This is a key technique for preparing functional NRs that can have practical applications.

Original language	English
Pages (from-to)	2-5
Number of pages	4
Journal	Langmuir
Volume	22
Issue number	1
DOIs	https://doi.org/10.1021/la0520029
Publication status	Published - Jan 3 2006

All Science Journal Classification (ASJC) codes

Materials Science(all)
Condensed Matter Physics
Surfaces and Interfaces
Spectroscopy
Electrochemistry

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10.1021/la0520029

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abstract = "Hexadecyltrimethylammonium bromide (CTAB), which is necessary for the preparation of gold nanorods (NRs), was extracted from a NR solution into a chloroform phase containing phosphatidylcholine (PC). After three extractions, the zeta potential of the NRs remained positive, but its magnitude decreased from+67 ±1 to+15± 1 mV. Transmission electron microscopy and energy-dispersive X-ray analysis indicated that the NRs were passivated with PC. The PC layer on the NR surface contributed to the prevention of NR aggregation. The PC-passivated NRs showed low cytotoxicity in comparison with twice-centrifuged NRs. It was shown that a negligible amount of CTAB was dispersed in the NR solution after the extraction. The extraction using a chloroform phase containing PC was found to be a convenient way of replacing the CTAB with alternative capping agents such as PC. This is a key technique for preparing functional NRs that can have practical applications.",

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T1 - Modification of gold nanorods using phosphatidylcholine to reduce cytotoxicity

AU - Takahashi, Hironobu

AU - Niidome, Yasuro

AU - Niidome, Takuro

AU - Kaneko, Kenji

AU - Kawasaki, Hideya

AU - Yamada, Sunao

PY - 2006/1/3

Y1 - 2006/1/3

N2 - Hexadecyltrimethylammonium bromide (CTAB), which is necessary for the preparation of gold nanorods (NRs), was extracted from a NR solution into a chloroform phase containing phosphatidylcholine (PC). After three extractions, the zeta potential of the NRs remained positive, but its magnitude decreased from+67 ±1 to+15± 1 mV. Transmission electron microscopy and energy-dispersive X-ray analysis indicated that the NRs were passivated with PC. The PC layer on the NR surface contributed to the prevention of NR aggregation. The PC-passivated NRs showed low cytotoxicity in comparison with twice-centrifuged NRs. It was shown that a negligible amount of CTAB was dispersed in the NR solution after the extraction. The extraction using a chloroform phase containing PC was found to be a convenient way of replacing the CTAB with alternative capping agents such as PC. This is a key technique for preparing functional NRs that can have practical applications.

AB - Hexadecyltrimethylammonium bromide (CTAB), which is necessary for the preparation of gold nanorods (NRs), was extracted from a NR solution into a chloroform phase containing phosphatidylcholine (PC). After three extractions, the zeta potential of the NRs remained positive, but its magnitude decreased from+67 ±1 to+15± 1 mV. Transmission electron microscopy and energy-dispersive X-ray analysis indicated that the NRs were passivated with PC. The PC layer on the NR surface contributed to the prevention of NR aggregation. The PC-passivated NRs showed low cytotoxicity in comparison with twice-centrifuged NRs. It was shown that a negligible amount of CTAB was dispersed in the NR solution after the extraction. The extraction using a chloroform phase containing PC was found to be a convenient way of replacing the CTAB with alternative capping agents such as PC. This is a key technique for preparing functional NRs that can have practical applications.

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Modification of gold nanorods using phosphatidylcholine to reduce cytotoxicity

Abstract

All Science Journal Classification (ASJC) codes

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