Abstract
In this work, we have developed a new technique to stabilize a ternary complex composed of plasmid DNA, a linear polyethyleneimine (LPEI) and serum albumin. A stearoyl group was conjugated to LPEI as a specific ligand for serum albumin. The resultant ternary complex has excellent stability in physiological saline conditions, maintaining its initial diameter and preventing aggregation of red blood cells. The ternary complex has equivalent transfection ability to and significantly lower cytotoxicity than unmodified LPEI. Therefore, the ternary complex is potentially useful as a new gene carrier, possessing high blood stability.
| Original language | English |
|---|---|
| Pages (from-to) | 1382-1393 |
| Number of pages | 12 |
| Journal | Journal of Biomaterials Science, Polymer Edition |
| Volume | 28 |
| Issue number | 13 |
| DOIs | |
| Publication status | Published - Sep 2 2017 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Bioengineering
- Biomaterials
- Biomedical Engineering
Cite this
Modification of ligands for serum albumin on polyethyleneimine to stabilize polyplexes in gene delivery. / Nakamura, Yuta; Sato, Hikari; Nobori, Takanobu; Matsumoto, Hotaru; Toyama, Shoko; Shuno, Tomohiro; Kishimura, Akihiro; Mori, Takeshi; Katayama, Yoshiki.
In: Journal of Biomaterials Science, Polymer Edition, Vol. 28, No. 13, 02.09.2017, p. 1382-1393.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Modification of ligands for serum albumin on polyethyleneimine to stabilize polyplexes in gene delivery
AU - Nakamura, Yuta
AU - Sato, Hikari
AU - Nobori, Takanobu
AU - Matsumoto, Hotaru
AU - Toyama, Shoko
AU - Shuno, Tomohiro
AU - Kishimura, Akihiro
AU - Mori, Takeshi
AU - Katayama, Yoshiki
PY - 2017/9/2
Y1 - 2017/9/2
N2 - In this work, we have developed a new technique to stabilize a ternary complex composed of plasmid DNA, a linear polyethyleneimine (LPEI) and serum albumin. A stearoyl group was conjugated to LPEI as a specific ligand for serum albumin. The resultant ternary complex has excellent stability in physiological saline conditions, maintaining its initial diameter and preventing aggregation of red blood cells. The ternary complex has equivalent transfection ability to and significantly lower cytotoxicity than unmodified LPEI. Therefore, the ternary complex is potentially useful as a new gene carrier, possessing high blood stability.
AB - In this work, we have developed a new technique to stabilize a ternary complex composed of plasmid DNA, a linear polyethyleneimine (LPEI) and serum albumin. A stearoyl group was conjugated to LPEI as a specific ligand for serum albumin. The resultant ternary complex has excellent stability in physiological saline conditions, maintaining its initial diameter and preventing aggregation of red blood cells. The ternary complex has equivalent transfection ability to and significantly lower cytotoxicity than unmodified LPEI. Therefore, the ternary complex is potentially useful as a new gene carrier, possessing high blood stability.
UR - http://www.scopus.com/inward/record.url?scp=85019728336&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85019728336&partnerID=8YFLogxK
U2 - 10.1080/09205063.2017.1328730
DO - 10.1080/09205063.2017.1328730
M3 - Article
C2 - 28485674
AN - SCOPUS:85019728336
VL - 28
SP - 1382
EP - 1393
JO - Journal of Biomaterials Science, Polymer Edition
JF - Journal of Biomaterials Science, Polymer Edition
SN - 0920-5063
IS - 13
ER -