Modification of ligands for serum albumin on polyethyleneimine to stabilize polyplexes in gene delivery

Yuta Nakamura; Hikari Sato; Takanobu Nobori; Hotaru Matsumoto; Shoko Toyama; Tomohiro Shuno; Akihiro Kishimura; Takeshi Mori; Yoshiki Katayama

doi:10.1080/09205063.2017.1328730

Modification of ligands for serum albumin on polyethyleneimine to stabilize polyplexes in gene delivery

Yuta Nakamura, Hikari Sato, Takanobu Nobori, Hotaru Matsumoto, Shoko Toyama, Tomohiro Shuno, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

In this work, we have developed a new technique to stabilize a ternary complex composed of plasmid DNA, a linear polyethyleneimine (LPEI) and serum albumin. A stearoyl group was conjugated to LPEI as a specific ligand for serum albumin. The resultant ternary complex has excellent stability in physiological saline conditions, maintaining its initial diameter and preventing aggregation of red blood cells. The ternary complex has equivalent transfection ability to and significantly lower cytotoxicity than unmodified LPEI. Therefore, the ternary complex is potentially useful as a new gene carrier, possessing high blood stability.

Original language	English
Pages (from-to)	1382-1393
Number of pages	12
Journal	Journal of Biomaterials Science, Polymer Edition
Volume	28
Issue number	13
DOIs	https://doi.org/10.1080/09205063.2017.1328730
Publication status	Published - Sep 2 2017

All Science Journal Classification (ASJC) codes

Biophysics
Bioengineering
Biomaterials
Biomedical Engineering

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Modification of ligands for serum albumin on polyethyleneimine to stabilize polyplexes in gene delivery. / Nakamura, Yuta; Sato, Hikari; Nobori, Takanobu; Matsumoto, Hotaru; Toyama, Shoko; Shuno, Tomohiro; Kishimura, Akihiro ; Mori, Takeshi ; Katayama, Yoshiki.

In: Journal of Biomaterials Science, Polymer Edition, Vol. 28, No. 13, 02.09.2017, p. 1382-1393.

Research output: Contribution to journal › Article › peer-review

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