Modification of ligands for serum albumin on polyethyleneimine to stabilize polyplexes in gene delivery

Yuta Nakamura, Hikari Sato, Takanobu Nobori, Hotaru Matsumoto, Shoko Toyama, Tomohiro Shuno, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

In this work, we have developed a new technique to stabilize a ternary complex composed of plasmid DNA, a linear polyethyleneimine (LPEI) and serum albumin. A stearoyl group was conjugated to LPEI as a specific ligand for serum albumin. The resultant ternary complex has excellent stability in physiological saline conditions, maintaining its initial diameter and preventing aggregation of red blood cells. The ternary complex has equivalent transfection ability to and significantly lower cytotoxicity than unmodified LPEI. Therefore, the ternary complex is potentially useful as a new gene carrier, possessing high blood stability.

Original languageEnglish
Pages (from-to)1382-1393
Number of pages12
JournalJournal of Biomaterials Science, Polymer Edition
Volume28
Issue number13
DOIs
Publication statusPublished - Sep 2 2017

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Modification of ligands for serum albumin on polyethyleneimine to stabilize polyplexes in gene delivery'. Together they form a unique fingerprint.

Cite this