Modification of plant bait technique for direct diagnosis for Rhizoctonia rice pathogens from Myanmar paddy field soils

Masaru Matsumoto, Myo Zaw, Seint San Aye

Research output: Contribution to journalArticle

Abstract

A modified baiting technique was conducted for selective isolation, fungal DNA diagnosis and fungal cell lipid assay derived from Myanmar isolates of Rhizoctonia spp., causal agents of rice sheath diseases by trapping selective plant stem segments. Bait plant materials of rice, mat rush and cotton were successfully used to isolate R. solani AG1-IA, R. oryzae and R. oryzae-sativae. Moreover, the three plant materials were also effectively used to detect genomic DNA derived from all Rhizoctonia spp. obtained from Myanmar. Rice segment was the most successful materials for detection of fungal cell lipids including palmitic, stearic and linoleic acids. The results of this experiment demonstrate that bait plant materials of rice, mat rush and cotton were the best useful tools for not only direct isolation, but also fungal DNA diagnosis and cell lipid assay of Myanmar soil environmental conditions.

Original languageEnglish
Pages (from-to)777-784
Number of pages8
JournalArchives of Phytopathology and Plant Protection
Volume48
Issue number13-16
DOIs
Publication statusPublished - Oct 2 2015

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Rhizoctonia
Myanmar
baits
paddies
rice
pathogens
Oryza
soil
DNA
cotton
lipids
baiting
assays
cells
stearic acid
methodology
palmitic acid
trapping
linoleic acid
genomics

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science

Cite this

Modification of plant bait technique for direct diagnosis for Rhizoctonia rice pathogens from Myanmar paddy field soils. / Matsumoto, Masaru; Zaw, Myo; Aye, Seint San.

In: Archives of Phytopathology and Plant Protection, Vol. 48, No. 13-16, 02.10.2015, p. 777-784.

Research output: Contribution to journalArticle

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