Modification of prostacyclin-stimulatory activity in sera by glucose, insulin, low density lipoprotein, linoleic acid and linoleic acid hydroperoxide

F. Umeda, M. Kunisaki, T. Inoguchi, H. Nawata

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    Reduced prostacyclin (PGI2) production by the vascular wall has been proposed as one of the possible causes of diabetic vascular complications. We found an activity which stimulated PGI2 production by cultured endothelial cells (PGI2-stimulatory activity, PSA) in human plasma-derived serum (PDS). The PSA was less in patients with diabetes mellitus. The present study was undertaken to evaluate how metabolic factors relevant to diabetic angiopathy modify the PSA. Pooled PDS was prepared from 10 healthy volunteers. The 6-keto-PGF (6KF, a stable metabolite of PGI2) production by cultured bovine aortic endothelial cells was maximally stimulated by Dulbecco's modified Eagle's medium (DMEM) containing 10% pooled PDS after incubation for 60 min. The production of 6KF was reduced in a dose-dependent manner by the addition of 10% pooled PDS with glucose and linoleic acid hydroperoxide (lipid peroxide). In contrast, human low density lipoprotein (LDL) and linoleic acid (unsaturated fatty acid) enhanced the production of 6KF by 10% pooled PDS in a dose-dependent manner. Insulin, however, showed no effect on the production of 6KF by 10% pooled PDS. These results suggest that the reduced PSA in diabetics may be the result, in part, of a modification of the PSA by diabetic metabolic factors such as glucose and lipid peroxide.

    Original languageEnglish
    Pages (from-to)137-144
    Number of pages8
    JournalDiabetes Research and Clinical Practice
    Volume8
    Issue number2
    DOIs
    Publication statusPublished - Jan 1990

    Fingerprint

    Epoprostenol
    Linoleic Acid
    LDL Lipoproteins
    Insulin
    Glucose
    Serum
    Diabetic Angiopathies
    Lipid Peroxides
    Endothelial Cells
    linoleic acid hydroperoxide
    Eagles
    Unsaturated Fatty Acids
    Human Activities
    Blood Vessels
    Cultured Cells
    Diabetes Mellitus
    Healthy Volunteers

    All Science Journal Classification (ASJC) codes

    • Internal Medicine
    • Endocrinology, Diabetes and Metabolism
    • Endocrinology

    Cite this

    Modification of prostacyclin-stimulatory activity in sera by glucose, insulin, low density lipoprotein, linoleic acid and linoleic acid hydroperoxide. / Umeda, F.; Kunisaki, M.; Inoguchi, T.; Nawata, H.

    In: Diabetes Research and Clinical Practice, Vol. 8, No. 2, 01.1990, p. 137-144.

    Research output: Contribution to journalArticle

    @article{964b1df379254145a9a397b84baa85c7,
    title = "Modification of prostacyclin-stimulatory activity in sera by glucose, insulin, low density lipoprotein, linoleic acid and linoleic acid hydroperoxide",
    abstract = "Reduced prostacyclin (PGI2) production by the vascular wall has been proposed as one of the possible causes of diabetic vascular complications. We found an activity which stimulated PGI2 production by cultured endothelial cells (PGI2-stimulatory activity, PSA) in human plasma-derived serum (PDS). The PSA was less in patients with diabetes mellitus. The present study was undertaken to evaluate how metabolic factors relevant to diabetic angiopathy modify the PSA. Pooled PDS was prepared from 10 healthy volunteers. The 6-keto-PGF1α (6KF, a stable metabolite of PGI2) production by cultured bovine aortic endothelial cells was maximally stimulated by Dulbecco's modified Eagle's medium (DMEM) containing 10{\%} pooled PDS after incubation for 60 min. The production of 6KF was reduced in a dose-dependent manner by the addition of 10{\%} pooled PDS with glucose and linoleic acid hydroperoxide (lipid peroxide). In contrast, human low density lipoprotein (LDL) and linoleic acid (unsaturated fatty acid) enhanced the production of 6KF by 10{\%} pooled PDS in a dose-dependent manner. Insulin, however, showed no effect on the production of 6KF by 10{\%} pooled PDS. These results suggest that the reduced PSA in diabetics may be the result, in part, of a modification of the PSA by diabetic metabolic factors such as glucose and lipid peroxide.",
    author = "F. Umeda and M. Kunisaki and T. Inoguchi and H. Nawata",
    year = "1990",
    month = "1",
    doi = "10.1016/0168-8227(90)90024-N",
    language = "English",
    volume = "8",
    pages = "137--144",
    journal = "Diabetes Research and Clinical Practice",
    issn = "0168-8227",
    publisher = "Elsevier Ireland Ltd",
    number = "2",

    }

    TY - JOUR

    T1 - Modification of prostacyclin-stimulatory activity in sera by glucose, insulin, low density lipoprotein, linoleic acid and linoleic acid hydroperoxide

    AU - Umeda, F.

    AU - Kunisaki, M.

    AU - Inoguchi, T.

    AU - Nawata, H.

    PY - 1990/1

    Y1 - 1990/1

    N2 - Reduced prostacyclin (PGI2) production by the vascular wall has been proposed as one of the possible causes of diabetic vascular complications. We found an activity which stimulated PGI2 production by cultured endothelial cells (PGI2-stimulatory activity, PSA) in human plasma-derived serum (PDS). The PSA was less in patients with diabetes mellitus. The present study was undertaken to evaluate how metabolic factors relevant to diabetic angiopathy modify the PSA. Pooled PDS was prepared from 10 healthy volunteers. The 6-keto-PGF1α (6KF, a stable metabolite of PGI2) production by cultured bovine aortic endothelial cells was maximally stimulated by Dulbecco's modified Eagle's medium (DMEM) containing 10% pooled PDS after incubation for 60 min. The production of 6KF was reduced in a dose-dependent manner by the addition of 10% pooled PDS with glucose and linoleic acid hydroperoxide (lipid peroxide). In contrast, human low density lipoprotein (LDL) and linoleic acid (unsaturated fatty acid) enhanced the production of 6KF by 10% pooled PDS in a dose-dependent manner. Insulin, however, showed no effect on the production of 6KF by 10% pooled PDS. These results suggest that the reduced PSA in diabetics may be the result, in part, of a modification of the PSA by diabetic metabolic factors such as glucose and lipid peroxide.

    AB - Reduced prostacyclin (PGI2) production by the vascular wall has been proposed as one of the possible causes of diabetic vascular complications. We found an activity which stimulated PGI2 production by cultured endothelial cells (PGI2-stimulatory activity, PSA) in human plasma-derived serum (PDS). The PSA was less in patients with diabetes mellitus. The present study was undertaken to evaluate how metabolic factors relevant to diabetic angiopathy modify the PSA. Pooled PDS was prepared from 10 healthy volunteers. The 6-keto-PGF1α (6KF, a stable metabolite of PGI2) production by cultured bovine aortic endothelial cells was maximally stimulated by Dulbecco's modified Eagle's medium (DMEM) containing 10% pooled PDS after incubation for 60 min. The production of 6KF was reduced in a dose-dependent manner by the addition of 10% pooled PDS with glucose and linoleic acid hydroperoxide (lipid peroxide). In contrast, human low density lipoprotein (LDL) and linoleic acid (unsaturated fatty acid) enhanced the production of 6KF by 10% pooled PDS in a dose-dependent manner. Insulin, however, showed no effect on the production of 6KF by 10% pooled PDS. These results suggest that the reduced PSA in diabetics may be the result, in part, of a modification of the PSA by diabetic metabolic factors such as glucose and lipid peroxide.

    UR - http://www.scopus.com/inward/record.url?scp=0025101791&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0025101791&partnerID=8YFLogxK

    U2 - 10.1016/0168-8227(90)90024-N

    DO - 10.1016/0168-8227(90)90024-N

    M3 - Article

    C2 - 2106424

    AN - SCOPUS:0025101791

    VL - 8

    SP - 137

    EP - 144

    JO - Diabetes Research and Clinical Practice

    JF - Diabetes Research and Clinical Practice

    SN - 0168-8227

    IS - 2

    ER -