TY - JOUR
T1 - Modified sympathetic regulation in N-type calcium channel null-mouse
AU - Murakami, Manabu
AU - Ohba, Takayoshi
AU - Wu, Tsai Wen
AU - Fujisawa, Susumu
AU - Suzuki, Takashi
AU - Takahashi, Yoichiro
AU - Takahashi, Eiki
AU - Watanabe, Hiroyuki
AU - Miyoshi, Ichiro
AU - Ono, Kyoichi
AU - Sasano, Hironobu
AU - Ito, Hiroshi
AU - Iijima, Toshihiko
N1 - Funding Information:
This research was sponsored partly by Grants-in-Aid from the Ministry of Education, Science, and Culture of Japan.
PY - 2007/3/23
Y1 - 2007/3/23
N2 - To elucidate the physiological importance of neuronal (N)-type calcium channels in sympathetic controls, we analyzed N-type channel-deficient (NKO) mice. Immunoprecipitation analysis revealed increased interaction between β3 (a major accessory subunit of N-type channels) and R-type channel-forming CaV2.3 in NKO mice. R-R intervals in NKO ECG recordings were elongated and fluctuating, suggesting disturbed sympathetic tonus. N-type channel inhibitors elongated the R-R interval in control mice, whereas R-type channel blocking with SNX-482 significantly affected NKO but not control mice, indicating a compensatory role for R-type channels. Echocardiography and Langendorff heart analysis confirmed a major role for R-type channels in NKO mice. Combined, our biochemical and physiological analyses strongly suggest that the remaining sympathetic tonus in NKO mice is dependent on R-type calcium channels.
AB - To elucidate the physiological importance of neuronal (N)-type calcium channels in sympathetic controls, we analyzed N-type channel-deficient (NKO) mice. Immunoprecipitation analysis revealed increased interaction between β3 (a major accessory subunit of N-type channels) and R-type channel-forming CaV2.3 in NKO mice. R-R intervals in NKO ECG recordings were elongated and fluctuating, suggesting disturbed sympathetic tonus. N-type channel inhibitors elongated the R-R interval in control mice, whereas R-type channel blocking with SNX-482 significantly affected NKO but not control mice, indicating a compensatory role for R-type channels. Echocardiography and Langendorff heart analysis confirmed a major role for R-type channels in NKO mice. Combined, our biochemical and physiological analyses strongly suggest that the remaining sympathetic tonus in NKO mice is dependent on R-type calcium channels.
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U2 - 10.1016/j.bbrc.2007.01.087
DO - 10.1016/j.bbrc.2007.01.087
M3 - Article
C2 - 17275790
AN - SCOPUS:33846807591
VL - 354
SP - 1016
EP - 1020
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -