Modified sympathetic regulation in N-type calcium channel null-mouse

Manabu Murakami, Takayoshi Ohba, Tsai Wen Wu, Susumu Fujisawa, Takashi Suzuki, Yoichiro Takahashi, Eiki Takahashi, Hiroyuki Watanabe, Ichiro Miyoshi, Kyoichi Ono, Hironobu Sasano, Hiroshi Ito, Toshihiko Iijima

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

To elucidate the physiological importance of neuronal (N)-type calcium channels in sympathetic controls, we analyzed N-type channel-deficient (NKO) mice. Immunoprecipitation analysis revealed increased interaction between β3 (a major accessory subunit of N-type channels) and R-type channel-forming CaV2.3 in NKO mice. R-R intervals in NKO ECG recordings were elongated and fluctuating, suggesting disturbed sympathetic tonus. N-type channel inhibitors elongated the R-R interval in control mice, whereas R-type channel blocking with SNX-482 significantly affected NKO but not control mice, indicating a compensatory role for R-type channels. Echocardiography and Langendorff heart analysis confirmed a major role for R-type channels in NKO mice. Combined, our biochemical and physiological analyses strongly suggest that the remaining sympathetic tonus in NKO mice is dependent on R-type calcium channels.

Original languageEnglish
Pages (from-to)1016-1020
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume354
Issue number4
DOIs
Publication statusPublished - Mar 23 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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