Modulation by alkyl p-hydroxybenzoates of voltage- and ligand-gated channels in peripheral neuronal cells

K. Inoue, K. Nakazawa, K. Inoue, K. Fujimori, A. Takanaka

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Effects of alkyl p-hydroxybenzoates (APHBs), which are used as preservatives, on ion channels were investigated in rat pheochromocytoma PC12 cells. Methyl p-hydroxybenzoate (MPHB; 300 μM) and butyul p-hydroxybenzoate (BPHB; 300 μM) inhibited Ba2+ current passing through Ca2+ channels, and facilitated the inactivation of the Ba2+ current. K+ current obtained with a depolarizing voltage-step was also suppressed by 300 μM MPHB or 300 μM BPHB. The extent of the suppression of the K+ current was not affected by extracellular Cd2+, suggesting that the suppression of the K+ current is not a secondary effect arising from the Ca2+ channel inhibition. An inward current activated by acetylcholine (ACh; 100 μM) was abolished by 300 μM BPHB, and it was partially blocked by 300 μM MPHB. In contrast to the ACh-activated current, an inward current activated by ATP (30 μM) was markedly potentiated by 300 μM BPHB. The results suggest that APHBs exert significant effects on the voltage- and ligand-gated channels. The significance of these channel modifications were discussed in relation to reported effects of APHBs, including induction of minor irritation.

Original languageEnglish
Pages (from-to)891-896
Number of pages6
JournalNeuropharmacology
Volume33
Issue number7
DOIs
Publication statusPublished - Jul 1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Cellular and Molecular Neuroscience

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