Modulation by alkyl p-hydroxybenzoates of voltage- and ligand-gated channels in peripheral neuronal cells

Kazuhide Inoue, K. Nakazawa, K. Inoue, K. Fujimori, A. Takanaka

Research output: Contribution to journalArticle

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Abstract

Effects of alkyl p-hydroxybenzoates (APHBs), which are used as preservatives, on ion channels were investigated in rat pheochromocytoma PC12 cells. Methyl p-hydroxybenzoate (MPHB; 300 μM) and butyul p-hydroxybenzoate (BPHB; 300 μM) inhibited Ba2+ current passing through Ca2+ channels, and facilitated the inactivation of the Ba2+ current. K+ current obtained with a depolarizing voltage-step was also suppressed by 300 μM MPHB or 300 μM BPHB. The extent of the suppression of the K+ current was not affected by extracellular Cd2+, suggesting that the suppression of the K+ current is not a secondary effect arising from the Ca2+ channel inhibition. An inward current activated by acetylcholine (ACh; 100 μM) was abolished by 300 μM BPHB, and it was partially blocked by 300 μM MPHB. In contrast to the ACh-activated current, an inward current activated by ATP (30 μM) was markedly potentiated by 300 μM BPHB. The results suggest that APHBs exert significant effects on the voltage- and ligand-gated channels. The significance of these channel modifications were discussed in relation to reported effects of APHBs, including induction of minor irritation.

Original languageEnglish
Pages (from-to)891-896
Number of pages6
JournalNeuropharmacology
Volume33
Issue number7
DOIs
Publication statusPublished - Jan 1 1994
Externally publishedYes

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Ligand-Gated Ion Channels
PC12 Cells
Pheochromocytoma
Ion Channels
Acetylcholine
Adenosine Triphosphate
4-hydroxybenzoic acid

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Cellular and Molecular Neuroscience

Cite this

Modulation by alkyl p-hydroxybenzoates of voltage- and ligand-gated channels in peripheral neuronal cells. / Inoue, Kazuhide; Nakazawa, K.; Inoue, K.; Fujimori, K.; Takanaka, A.

In: Neuropharmacology, Vol. 33, No. 7, 01.01.1994, p. 891-896.

Research output: Contribution to journalArticle

Inoue, Kazuhide ; Nakazawa, K. ; Inoue, K. ; Fujimori, K. ; Takanaka, A. / Modulation by alkyl p-hydroxybenzoates of voltage- and ligand-gated channels in peripheral neuronal cells. In: Neuropharmacology. 1994 ; Vol. 33, No. 7. pp. 891-896.
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