Modulation of glutamate-induced outward current by prostaglandin E 2 in rat dissociated preoptic neurons

Toshihiko Katafuchi, Shumin Duan, Sachiko Take, Megumu Yoshimura

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The preoptic/anterior hypothalamus (POA) is one of the major brain regions where cytokines and their related mediators (i.e., prostaglandins) exert diverse actions. In the present study, the modulatory effects of prostaglandin E 2 (PGE2) on the glutamate-induced membrane currents were examined using perforated-patch clamp method in rat POA neurons that had been mechanically dissociated by vibration without enzyme treatment. Application of glutamate through U-tube induced a slow outward current following fast inward ionotroic current at a holding membrane potential of -30 mV. The slow outward current was also induced by N-methyl-d-aspartate (NMDA), accompanied by an increased membrane conductance, and inhibited by perfusion with Ca 2+-free solution, tetraethylammonium chloride (TEA), and apamin, suggesting a Ca2+-dependent K+ current (KCa) activated by Ca2+ entry through NMDA channels. Perfusion with PGE2 at 0.1-10 μM, a principal mediator of fever and neuroendocrine control at the POA, did not produce apparent current by itself, but selectively potentiated the glutamate- or NMDA-induced KCa without affecting inward currents. The KCa induced by activation of NMDA receptors may serve as a feedback mechanism and the modulatory effects of PGE2 on the KCa may have an important physiological significance.

Original languageEnglish
Pages (from-to)180-186
Number of pages7
JournalBrain Research
Volume1037
Issue number1-2
DOIs
Publication statusPublished - Mar 10 2005

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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