Modulation of glycine-induced currents by zinc and other metal cations in neurons acutely dissociated from the dorsal motor nucleus of the vagus of the rat

Atsushi Doi, Kiyonori Kishimoto, Hitoshi Ishibashi

Research output: Contribution to journalArticle

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Abstract

Effects of Zn2+ and other polyvalent cations on glycine-induced currents in the freshly dissociated rat dorsal motor nucleus of the vagus neurons were investigated under voltage-clamp conditions by the use of the nystatin-perforated patch recording configuration. Glycine evoked a Cl- current in a concentration-dependent manner with a half-maximum effective concentration (EC50) of 3.3 x 10-5 M. Strychnine inhibited the 3 x 10- 5 M glycine-induced current in a concentration-dependent manner with a half- maximal inhibitory concentration (IC50) of 6.8 x 10-7 M. At low concentrations (3 x 10-8 M-3 x 10-6 M), Zn2+ potentiated the current elicited by 3 x 10-5 M glycine. On the other hand, at concentrations higher than 10-5 M, Zn2+ inhibited the glycine response. The biphasic action of Zn2+ was mimicked by Ni2+, but La3+ and Co2+ had only potentiating effect. Zn2+ shifted the concentration-response curve for the glycine- induced current without changing the maximum response, and the EC50 values for the glycine response in the absence and presence of 10-6 M and 10-4 M Zn2+ were 3.3 x 10-5 M, 1.3 x 10-5 M and 1.3 x 10-4 M, respectively. These results suggest that the biphasic modulation of glycine response by Zn2+ results from changes in apparent glycine affinity.

Original languageEnglish
Pages (from-to)424-430
Number of pages7
JournalBrain Research
Volume816
Issue number2
DOIs
Publication statusPublished - Jan 23 1999

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Glycine
Cations
Zinc
Metals
Neurons
Nystatin
Strychnine
Inhibitory Concentration 50

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Modulation of glycine-induced currents by zinc and other metal cations in neurons acutely dissociated from the dorsal motor nucleus of the vagus of the rat. / Doi, Atsushi; Kishimoto, Kiyonori; Ishibashi, Hitoshi.

In: Brain Research, Vol. 816, No. 2, 23.01.1999, p. 424-430.

Research output: Contribution to journalArticle

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