Abstract
Human telomeric DNA, d(GGGTTA)n, tends to adopt complex intramolecular G-quadruplex structure in solution. The sequence folds into G-quadruplex with the antiparallel orientation in the presence of sodium ions, while antiparallel and parallel conformations were coexisted in solution containing relatively high concentration of potassium ions. Here, we describe that cationic comb-type copolymer, poly(l-lysine)-graft-dextran (PLL-g-Dex), triggers selective folding of d[GGG(TTAGGG)3] into G-quadruplex with the parallel orientation. The parallel G-quadruplex formed in the presence of the copolymer has the melting temperature > 90 degrees C, including strong stabilization effect of the copolymer.
| Original language | English |
|---|---|
| Pages (from-to) | 53-54 |
| Number of pages | 2 |
| Journal | Nucleic acids symposium series (2004) |
| Issue number | 49 |
| Publication status | Published - 2005 |
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All Science Journal Classification (ASJC) codes
- Medicine(all)
Cite this
Modulation of highly ordered structures of human telomeric sequence by cationic copolymers. / Makita, Naoki; Kano, Arihiro; Yamayoshi, Asako; Akaike, Toshihiro; Maruyama, Atsushi.
In: Nucleic acids symposium series (2004), No. 49, 2005, p. 53-54.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Modulation of highly ordered structures of human telomeric sequence by cationic copolymers.
AU - Makita, Naoki
AU - Kano, Arihiro
AU - Yamayoshi, Asako
AU - Akaike, Toshihiro
AU - Maruyama, Atsushi
PY - 2005
Y1 - 2005
N2 - Human telomeric DNA, d(GGGTTA)n, tends to adopt complex intramolecular G-quadruplex structure in solution. The sequence folds into G-quadruplex with the antiparallel orientation in the presence of sodium ions, while antiparallel and parallel conformations were coexisted in solution containing relatively high concentration of potassium ions. Here, we describe that cationic comb-type copolymer, poly(l-lysine)-graft-dextran (PLL-g-Dex), triggers selective folding of d[GGG(TTAGGG)3] into G-quadruplex with the parallel orientation. The parallel G-quadruplex formed in the presence of the copolymer has the melting temperature > 90 degrees C, including strong stabilization effect of the copolymer.
AB - Human telomeric DNA, d(GGGTTA)n, tends to adopt complex intramolecular G-quadruplex structure in solution. The sequence folds into G-quadruplex with the antiparallel orientation in the presence of sodium ions, while antiparallel and parallel conformations were coexisted in solution containing relatively high concentration of potassium ions. Here, we describe that cationic comb-type copolymer, poly(l-lysine)-graft-dextran (PLL-g-Dex), triggers selective folding of d[GGG(TTAGGG)3] into G-quadruplex with the parallel orientation. The parallel G-quadruplex formed in the presence of the copolymer has the melting temperature > 90 degrees C, including strong stabilization effect of the copolymer.
UR - http://www.scopus.com/inward/record.url?scp=39049187993&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=39049187993&partnerID=8YFLogxK
M3 - Article
C2 - 17150629
AN - SCOPUS:39049187993
SP - 53
EP - 54
JO - Nucleic acids symposium series (2004)
JF - Nucleic acids symposium series (2004)
SN - 1746-8272
IS - 49
ER -