Modulation of the osteoconductive property and immune response of poly(ether ether ketone) by modification with calcium ions

R. Toita, Sunarso, A. N. Rashid, K. Tsuru, K. Ishikawa

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Both bone-forming cells and immune cells have pivotal roles in bone tissue repair. In this work, we prepared a Ca-modified poly(ether ether ketone) (PEEK) to enhance the osteoconductivity but mitigate immune response of cells. To modify Ca ions onto a chemically inert PEEK, PEEK was first coated with poly(norepinephrine) followed by soaking in Ca(OH)2 aqueous solution. Modification of Ca ions onto PEEK enhanced the proliferation and differentiation of MC3T3-E1 preosteoblast cells and human mesenchymal stem cells, but had no apparent effect on MLC-6 osteoclast-like cells. Naïve RAW264.7 macrophages cultured on pristine PEEK produced proinflammatory cytokines, but not on Ca-modified PEEK. Lipopolysaccharide-stimulated RAW264.7 macrophages on Ca-modified PEEK produced lower levels of proinflammatory cytokines but higher levels of anti-inflammatory cytokines compared with those on pristine PEEK. Lower levels of proinflammatory cytokines on Ca-modified PEEK were partially owing to higher interleukin-10 production mediated by Ca-activated pathways. Simple Ca modification of PEEK was shown to activate osteoblastic cell proliferation and differentiation, and shift macrophages towards an anti-inflammatory/wound healing type, which would be useful not only for bone tissue implants but also other implanted biomaterials.

Original languageEnglish
Pages (from-to)8738-8746
Number of pages9
JournalJournal of Materials Chemistry B
Volume3
Issue number44
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

Polyether ether ketones
Calcium
Modulation
Ions
Macrophages
Cytokines
Bone
Anti-Inflammatory Agents
Norepinephrine
Tissue
Cell proliferation
Biocompatible Materials
Stem cells
Biomaterials
Interleukin-10
Lipopolysaccharides
Repair

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biomedical Engineering
  • Materials Science(all)

Cite this

Modulation of the osteoconductive property and immune response of poly(ether ether ketone) by modification with calcium ions. / Toita, R.; Sunarso; Rashid, A. N.; Tsuru, K.; Ishikawa, K.

In: Journal of Materials Chemistry B, Vol. 3, No. 44, 01.01.2015, p. 8738-8746.

Research output: Contribution to journalArticle

@article{17f7783df897492193a7bb0a2be77891,
title = "Modulation of the osteoconductive property and immune response of poly(ether ether ketone) by modification with calcium ions",
abstract = "Both bone-forming cells and immune cells have pivotal roles in bone tissue repair. In this work, we prepared a Ca-modified poly(ether ether ketone) (PEEK) to enhance the osteoconductivity but mitigate immune response of cells. To modify Ca ions onto a chemically inert PEEK, PEEK was first coated with poly(norepinephrine) followed by soaking in Ca(OH)2 aqueous solution. Modification of Ca ions onto PEEK enhanced the proliferation and differentiation of MC3T3-E1 preosteoblast cells and human mesenchymal stem cells, but had no apparent effect on MLC-6 osteoclast-like cells. Na{\"i}ve RAW264.7 macrophages cultured on pristine PEEK produced proinflammatory cytokines, but not on Ca-modified PEEK. Lipopolysaccharide-stimulated RAW264.7 macrophages on Ca-modified PEEK produced lower levels of proinflammatory cytokines but higher levels of anti-inflammatory cytokines compared with those on pristine PEEK. Lower levels of proinflammatory cytokines on Ca-modified PEEK were partially owing to higher interleukin-10 production mediated by Ca-activated pathways. Simple Ca modification of PEEK was shown to activate osteoblastic cell proliferation and differentiation, and shift macrophages towards an anti-inflammatory/wound healing type, which would be useful not only for bone tissue implants but also other implanted biomaterials.",
author = "R. Toita and Sunarso and Rashid, {A. N.} and K. Tsuru and K. Ishikawa",
year = "2015",
month = "1",
day = "1",
doi = "10.1039/c5tb01679g",
language = "English",
volume = "3",
pages = "8738--8746",
journal = "Journal of Materials Chemistry B",
issn = "2050-7518",
publisher = "Royal Society of Chemistry",
number = "44",

}

TY - JOUR

T1 - Modulation of the osteoconductive property and immune response of poly(ether ether ketone) by modification with calcium ions

AU - Toita, R.

AU - Sunarso,

AU - Rashid, A. N.

AU - Tsuru, K.

AU - Ishikawa, K.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Both bone-forming cells and immune cells have pivotal roles in bone tissue repair. In this work, we prepared a Ca-modified poly(ether ether ketone) (PEEK) to enhance the osteoconductivity but mitigate immune response of cells. To modify Ca ions onto a chemically inert PEEK, PEEK was first coated with poly(norepinephrine) followed by soaking in Ca(OH)2 aqueous solution. Modification of Ca ions onto PEEK enhanced the proliferation and differentiation of MC3T3-E1 preosteoblast cells and human mesenchymal stem cells, but had no apparent effect on MLC-6 osteoclast-like cells. Naïve RAW264.7 macrophages cultured on pristine PEEK produced proinflammatory cytokines, but not on Ca-modified PEEK. Lipopolysaccharide-stimulated RAW264.7 macrophages on Ca-modified PEEK produced lower levels of proinflammatory cytokines but higher levels of anti-inflammatory cytokines compared with those on pristine PEEK. Lower levels of proinflammatory cytokines on Ca-modified PEEK were partially owing to higher interleukin-10 production mediated by Ca-activated pathways. Simple Ca modification of PEEK was shown to activate osteoblastic cell proliferation and differentiation, and shift macrophages towards an anti-inflammatory/wound healing type, which would be useful not only for bone tissue implants but also other implanted biomaterials.

AB - Both bone-forming cells and immune cells have pivotal roles in bone tissue repair. In this work, we prepared a Ca-modified poly(ether ether ketone) (PEEK) to enhance the osteoconductivity but mitigate immune response of cells. To modify Ca ions onto a chemically inert PEEK, PEEK was first coated with poly(norepinephrine) followed by soaking in Ca(OH)2 aqueous solution. Modification of Ca ions onto PEEK enhanced the proliferation and differentiation of MC3T3-E1 preosteoblast cells and human mesenchymal stem cells, but had no apparent effect on MLC-6 osteoclast-like cells. Naïve RAW264.7 macrophages cultured on pristine PEEK produced proinflammatory cytokines, but not on Ca-modified PEEK. Lipopolysaccharide-stimulated RAW264.7 macrophages on Ca-modified PEEK produced lower levels of proinflammatory cytokines but higher levels of anti-inflammatory cytokines compared with those on pristine PEEK. Lower levels of proinflammatory cytokines on Ca-modified PEEK were partially owing to higher interleukin-10 production mediated by Ca-activated pathways. Simple Ca modification of PEEK was shown to activate osteoblastic cell proliferation and differentiation, and shift macrophages towards an anti-inflammatory/wound healing type, which would be useful not only for bone tissue implants but also other implanted biomaterials.

UR - http://www.scopus.com/inward/record.url?scp=84946605428&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84946605428&partnerID=8YFLogxK

U2 - 10.1039/c5tb01679g

DO - 10.1039/c5tb01679g

M3 - Article

AN - SCOPUS:84946605428

VL - 3

SP - 8738

EP - 8746

JO - Journal of Materials Chemistry B

JF - Journal of Materials Chemistry B

SN - 2050-7518

IS - 44

ER -