Modulation of UDP-glucuronosyltransferase function by cytochrome P450

Evidence for the alteration of UGT2B7-catalyzed glucuronidation of morphine by CYP3A4

Shuso Takeda, Yuji Ishii, Megumi Iwanaga, Peter I. Mackenzie, Kiyoshi Nagata, Yasushi Yamazoe, Kazuta Oguri, Hideyuki Yamada

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Modulation of UDP-glucuronosyltransferase 2B7 (UGT2B7)-catalyzed morphine glucuronidation by cytochrome P450 (P450) was studied. The effects of P450 isozymes on the kinetic parameters of UGT2B7-catalyzed glucuronidation of the morphine 3-hydroxyl group were examined by simultaneous expression of UGT2B7 and either CYP3A4, -1A2, or -2C9 in COS-1 cells. Although coexpression of CYP3A4 with UGT2B7 had little effect on Vmax, the Km was increased by about 9.8-fold compared with the UGT2B7 single expression system. The other P450 isozymes (CYP1A2 and CYP2C9) had some effects on Km and Vmax values. Immunoprecipitation of UGT from solubilized human liver microsomes resulted in coprecipitation of CYP3A4 with UGT2B7. The protein-protein interaction between CYP3A4 and UGT2B7 was further confirmed by overlay assay using glutathione S-transferase-CYP3A4 fusion protein. Addition of CYP3A4 to untreated COS microsomes expressing UGT2B7 had no or minor effects on morphine glucuronidation. In contrast, the formation of morphine-3-glucuronide by detergent-treated microsomes from COS-1 cells expressing UGT2B7 was reduced by CYP3A4, whereas the formation of the 6-glucuronide was enhanced. These results strongly suggest that 1) the glucuronidation activity of UGT2B7 toward morphine is specifically modulated by interaction with CYP3A4 in microsomal membranes and that 2) CYP3A4 alters UGT2B7 regioselectivity so that the ratio of morphine activation/detoxication is increased. This study provides the first evidence that P450 is not only involved in oxidation of drugs but also modulates the function of UGTs.

Original languageEnglish
Pages (from-to)665-672
Number of pages8
JournalMolecular Pharmacology
Volume67
Issue number3
DOIs
Publication statusPublished - Mar 1 2005

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Cytochrome P-450 CYP3A
Glucuronosyltransferase
Cytochrome P-450 Enzyme System
Morphine
COS Cells
Microsomes
Isoenzymes
Cytochrome P-450 CYP1A2
Proteins
Glucuronides
Liver Microsomes
Glutathione Transferase
Immunoprecipitation
Detergents
Hydroxyl Radical

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology

Cite this

Modulation of UDP-glucuronosyltransferase function by cytochrome P450 : Evidence for the alteration of UGT2B7-catalyzed glucuronidation of morphine by CYP3A4. / Takeda, Shuso; Ishii, Yuji; Iwanaga, Megumi; Mackenzie, Peter I.; Nagata, Kiyoshi; Yamazoe, Yasushi; Oguri, Kazuta; Yamada, Hideyuki.

In: Molecular Pharmacology, Vol. 67, No. 3, 01.03.2005, p. 665-672.

Research output: Contribution to journalArticle

Takeda, Shuso ; Ishii, Yuji ; Iwanaga, Megumi ; Mackenzie, Peter I. ; Nagata, Kiyoshi ; Yamazoe, Yasushi ; Oguri, Kazuta ; Yamada, Hideyuki. / Modulation of UDP-glucuronosyltransferase function by cytochrome P450 : Evidence for the alteration of UGT2B7-catalyzed glucuronidation of morphine by CYP3A4. In: Molecular Pharmacology. 2005 ; Vol. 67, No. 3. pp. 665-672.
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