Abstract
The Pseudomonas aeruginosa ctx gene encoding cytotoxin is carried by a temperate phage φCTX. The genome of φCTX is a 35.5 kb double‐stranded DNA with cohesive ends (cos). It is unique in that the ctx gene and attP site of φCTX exist very close to the respective cohesive ends. In this study, we determined the structure of this attP–cos–ctx region. The termini of φCTX are 21‐base 5′ extended‐single‐stranded DNAs. The ctxgene is located 361 bp downstream of the left end (cosL). The attP core sequence of 30 bp exists only 647 bp apart from the right end (cosR). The attP–cos–ctx region contains six kinds of repeats and integration host factor‐binding sequences and showed sequence‐directed static bends, suggesting its potential to form a highly ordered structure. In addition, φCTX was found to integrate into the serine tRNA gene which was mapped to the 43–45 min region on the P. aeruginosachromosome.
| Original language | English |
|---|---|
| Pages (from-to) | 657-667 |
| Number of pages | 11 |
| Journal | Molecular Microbiology |
| Volume | 7 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Jan 1 1993 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Microbiology
- Molecular Biology
Cite this
Molecular analysis of a cytotoxin‐converting phage, φCTX, of Pseudomonas aeruginosa : structure of the attP–cos–ctx region and integration into the serine tRNA gene. / Hayashi, Tetsuya; Matsumoto, Hideki; Ohnishi, Makoto; Terawaki, Yoshiro.
In: Molecular Microbiology, Vol. 7, No. 5, 01.01.1993, p. 657-667.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular analysis of a cytotoxin‐converting phage, φCTX, of Pseudomonas aeruginosa
T2 - structure of the attP–cos–ctx region and integration into the serine tRNA gene
AU - Hayashi, Tetsuya
AU - Matsumoto, Hideki
AU - Ohnishi, Makoto
AU - Terawaki, Yoshiro
PY - 1993/1/1
Y1 - 1993/1/1
N2 - The Pseudomonas aeruginosa ctx gene encoding cytotoxin is carried by a temperate phage φCTX. The genome of φCTX is a 35.5 kb double‐stranded DNA with cohesive ends (cos). It is unique in that the ctx gene and attP site of φCTX exist very close to the respective cohesive ends. In this study, we determined the structure of this attP–cos–ctx region. The termini of φCTX are 21‐base 5′ extended‐single‐stranded DNAs. The ctxgene is located 361 bp downstream of the left end (cosL). The attP core sequence of 30 bp exists only 647 bp apart from the right end (cosR). The attP–cos–ctx region contains six kinds of repeats and integration host factor‐binding sequences and showed sequence‐directed static bends, suggesting its potential to form a highly ordered structure. In addition, φCTX was found to integrate into the serine tRNA gene which was mapped to the 43–45 min region on the P. aeruginosachromosome.
AB - The Pseudomonas aeruginosa ctx gene encoding cytotoxin is carried by a temperate phage φCTX. The genome of φCTX is a 35.5 kb double‐stranded DNA with cohesive ends (cos). It is unique in that the ctx gene and attP site of φCTX exist very close to the respective cohesive ends. In this study, we determined the structure of this attP–cos–ctx region. The termini of φCTX are 21‐base 5′ extended‐single‐stranded DNAs. The ctxgene is located 361 bp downstream of the left end (cosL). The attP core sequence of 30 bp exists only 647 bp apart from the right end (cosR). The attP–cos–ctx region contains six kinds of repeats and integration host factor‐binding sequences and showed sequence‐directed static bends, suggesting its potential to form a highly ordered structure. In addition, φCTX was found to integrate into the serine tRNA gene which was mapped to the 43–45 min region on the P. aeruginosachromosome.
UR - http://www.scopus.com/inward/record.url?scp=0027529352&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027529352&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2958.1993.tb01157.x
DO - 10.1111/j.1365-2958.1993.tb01157.x
M3 - Article
C2 - 8469112
AN - SCOPUS:0027529352
VL - 7
SP - 657
EP - 667
JO - Molecular Microbiology
JF - Molecular Microbiology
SN - 0950-382X
IS - 5
ER -