Molecular analysis of a cytotoxin‐converting phage, φCTX, of Pseudomonas aeruginosa: structure of the attP–cos–ctx region and integration into the serine tRNA gene

Tetsuya Hayashi, Hideki Matsumoto, Makoto Ohnishi, Yoshiro Terawaki

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The Pseudomonas aeruginosa ctx gene encoding cytotoxin is carried by a temperate phage φCTX. The genome of φCTX is a 35.5 kb double‐stranded DNA with cohesive ends (cos). It is unique in that the ctx gene and attP site of φCTX exist very close to the respective cohesive ends. In this study, we determined the structure of this attP–cos–ctx region. The termini of φCTX are 21‐base 5′ extended‐single‐stranded DNAs. The ctxgene is located 361 bp downstream of the left end (cosL). The attP core sequence of 30 bp exists only 647 bp apart from the right end (cosR). The attP–cos–ctx region contains six kinds of repeats and integration host factor‐binding sequences and showed sequence‐directed static bends, suggesting its potential to form a highly ordered structure. In addition, φCTX was found to integrate into the serine tRNA gene which was mapped to the 43–45 min region on the P. aeruginosachromosome.

Original languageEnglish
Pages (from-to)657-667
Number of pages11
JournalMolecular Microbiology
Volume7
Issue number5
DOIs
Publication statusPublished - Mar 1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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