Molecular analysis of a new insertion sequence from Actinobacillus (Haemophilus) actinomycetemcomitans FDC Y4

Hideaki Hayashida, Hitoshi Hotokezaka, Naoya Ohara, Makoto Kimura, Okiuji Takagi, Takeshi Yamada

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have found a new insertion sequence (IS), designated ISAa1, downstream of the S10 operon in Actinobacillus (Haemophilus) actinomycetemcomitans FDC Y4. ISAa1, the first IS element characterized in this organism, is 705 bp long and lacks terminal inverted repeats. This element displayed significant homology with IS200. Hybridization patterns of genomic DNA of seven A. actinomycetemcomitans strains with an internal ISAa1 probe varied depending on the serotypes, suggesting that ISAa1 might be a useful tool for epidemiological studies.

Original languageEnglish
Pages (from-to)2449-2452
Number of pages4
JournalMicrobiology
Volume142
Issue number9
DOIs
Publication statusPublished - Jan 1 1996

Fingerprint

Aggregatibacter actinomycetemcomitans
Insertional Mutagenesis
S 10
Nucleic Acid Hybridization
DNA Transposable Elements
Terminal Repeat Sequences
Operon
Epidemiologic Studies
DNA
Serogroup

All Science Journal Classification (ASJC) codes

  • Microbiology

Cite this

Hayashida, H., Hotokezaka, H., Ohara, N., Kimura, M., Takagi, O., & Yamada, T. (1996). Molecular analysis of a new insertion sequence from Actinobacillus (Haemophilus) actinomycetemcomitans FDC Y4. Microbiology, 142(9), 2449-2452. https://doi.org/10.1099/00221287-142-9-2449

Molecular analysis of a new insertion sequence from Actinobacillus (Haemophilus) actinomycetemcomitans FDC Y4. / Hayashida, Hideaki; Hotokezaka, Hitoshi; Ohara, Naoya; Kimura, Makoto; Takagi, Okiuji; Yamada, Takeshi.

In: Microbiology, Vol. 142, No. 9, 01.01.1996, p. 2449-2452.

Research output: Contribution to journalArticle

Hayashida, H, Hotokezaka, H, Ohara, N, Kimura, M, Takagi, O & Yamada, T 1996, 'Molecular analysis of a new insertion sequence from Actinobacillus (Haemophilus) actinomycetemcomitans FDC Y4', Microbiology, vol. 142, no. 9, pp. 2449-2452. https://doi.org/10.1099/00221287-142-9-2449
Hayashida, Hideaki ; Hotokezaka, Hitoshi ; Ohara, Naoya ; Kimura, Makoto ; Takagi, Okiuji ; Yamada, Takeshi. / Molecular analysis of a new insertion sequence from Actinobacillus (Haemophilus) actinomycetemcomitans FDC Y4. In: Microbiology. 1996 ; Vol. 142, No. 9. pp. 2449-2452.
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