Molecular analysis of ATP-sensitive K+ channel subunits expressed in mouse vas deferens myocytes

Kazuomi Iwasa, Hai Lei Zhu, Atsushi Shibata, Yoshihiko Maehara, Noriyoshi Teramoto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background and Purpose ATP-sensitive K+ (KATP) channels, which are composed of KIR6.x associated with sulphonylurea receptor (SUR) subunits, have been detected in native smooth muscle cells, but it is currently not known which of these is expressed in mouse vas deferens myocytes. Experimental Approach Pharmacological and electrophysiological properties of KATP channels in mouse vas deferens myocytes were investigated using patch clamp techniques. Molecular biological analyses were performed to examine the properties of these KATP channels. Key Results During conventional whole-cell recording, pinacidil elicited an inward current that was suppressed by glibenclamide, a sulfonylurea agent, and by U-37883A, a selective KIR6.1 blocker. When 0.3 mM ATP was added to the pipette solution, the peak amplitude of the pinacidil-induced current was much smaller than that recorded in its absence. When 3 mM UDP, GDP or ADP was included in the pipette solution, an inward current was elicited after establishment of the conventional whole-cell configuration, with potency order being UDP > GDP > ADP. These nucleoside diphosphate-induced inward currents were suppressed by glibenclamide. MCC-134, a SUR modulator, induced glibenclamide-sensitive KATP currents that were similar to those induced by 100 μM pinacidil. In the cell-attached configuration, pinacidil activated channels with a conductance similar to that of KIR6.1. Reverse transcription PCR analysis revealed the expression of KIR6.1 and SUR2B transcripts and immunohistochemical studies indicated the presence of KIR6.1 and SUR2B proteins in the myocytes. Conclusions and Implications Our results indicate that native KATP channels in mouse vas deferens myocytes are a heterocomplex of KIR6.1 channels and SUR2B subunits.

Original languageEnglish
Pages (from-to)145-157
Number of pages13
JournalBritish Journal of Pharmacology
Volume171
Issue number1
DOIs
Publication statusPublished - Jan 1 2014

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Pinacidil
KATP Channels
Vas Deferens
Muscle Cells
Glyburide
Adenosine Triphosphate
Sulfonylurea Receptors
Uridine Diphosphate
Patch-Clamp Techniques
Adenosine Diphosphate
Diphosphates
Nucleosides
Reverse Transcription
Smooth Muscle Myocytes
Pharmacology
Polymerase Chain Reaction
Proteins

All Science Journal Classification (ASJC) codes

  • Pharmacology

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Molecular analysis of ATP-sensitive K+ channel subunits expressed in mouse vas deferens myocytes. / Iwasa, Kazuomi; Zhu, Hai Lei; Shibata, Atsushi; Maehara, Yoshihiko; Teramoto, Noriyoshi.

In: British Journal of Pharmacology, Vol. 171, No. 1, 01.01.2014, p. 145-157.

Research output: Contribution to journalArticle

Iwasa, Kazuomi ; Zhu, Hai Lei ; Shibata, Atsushi ; Maehara, Yoshihiko ; Teramoto, Noriyoshi. / Molecular analysis of ATP-sensitive K+ channel subunits expressed in mouse vas deferens myocytes. In: British Journal of Pharmacology. 2014 ; Vol. 171, No. 1. pp. 145-157.
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