Molecular analysis of fungal populations in patients with oral candidiasis using internal transcribed spacer region

Shinsuke Ieda, Masafumi Moriyama, Toru Takashita, takashi maehara, Yumi Imabayashi, Shoichi Shinozaki, Akihiko Tanaka, Hayashida Jun-Nosuke, Sachiko Furukawa, Miho Ohta, Yoshihisa Yamashita, Seiji Nakamura

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Oral candidiasis is closely associated with changes in the oral fungal flora and is caused primarily by Candida albicans. Conventional methods of fungal culture are time-consuming and not always conclusive. However, molecular genetic analysis of internal transcribed spacer (ITS) regions of fungal rRNA is rapid, reproducible and simple to perform. In this study we examined the fungal flora in patients with oral candidiasis and investigated changes in the flora after antifungal treatment using length heterogeneity-polymerization chain reaction (LH-PCR) analysis of ITS regions. Fifty-two patients with pseudomembranous oral candidiasis (POC) and 30 healthy controls were included in the study. Fungal DNA from oral rinse was examined for fungal species diversity by LH-PCR. Fungal populations were quantified by real-time PCR and previouslyunidentified signals were confirmed by nucleotide sequencing. Relationships between the oral fungal flora and treatmentresistant factors were also examined. POC patients showed significantly more fungal species and a greater density of fungi than control individuals. Sixteen fungi were newly identified. The fungal populations from both groups were composed predominantly of C. albicans, though the ratio of C. dubliniensis was significantly higher in POC patients than in controls. The diversity and density of fungi were significantly reduced after treatment. Furthermore, fungal diversity and the proportion of C. dubliniensis were positively correlated with treatment duration. These results suggest that C. dubliniensis and high fungal flora diversity might be involved in the pathogenesis of oral candidiasis. We therefore conclude that LH-PCR is a useful technique for diagnosing and assessing the severity of oral candidal infection.

Original languageEnglish
Article numbere101156
JournalPloS one
Volume9
Issue number6
DOIs
Publication statusPublished - Jun 30 2014

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Oral Candidiasis
candidiasis
Fungi
internal transcribed spacers
mouth
Polymerization
Fungal DNA
Population
Candida
Biodiversity
Candida albicans
polymerization
Nucleotides
fungi
Population Groups
Real-Time Polymerase Chain Reaction
Molecular Biology
Therapeutics
molecular genetics
genetic techniques and protocols

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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Molecular analysis of fungal populations in patients with oral candidiasis using internal transcribed spacer region. / Ieda, Shinsuke; Moriyama, Masafumi; Takashita, Toru; maehara, takashi; Imabayashi, Yumi; Shinozaki, Shoichi; Tanaka, Akihiko; Jun-Nosuke, Hayashida; Furukawa, Sachiko; Ohta, Miho; Yamashita, Yoshihisa; Nakamura, Seiji.

In: PloS one, Vol. 9, No. 6, e101156, 30.06.2014.

Research output: Contribution to journalArticle

Ieda, Shinsuke ; Moriyama, Masafumi ; Takashita, Toru ; maehara, takashi ; Imabayashi, Yumi ; Shinozaki, Shoichi ; Tanaka, Akihiko ; Jun-Nosuke, Hayashida ; Furukawa, Sachiko ; Ohta, Miho ; Yamashita, Yoshihisa ; Nakamura, Seiji. / Molecular analysis of fungal populations in patients with oral candidiasis using internal transcribed spacer region. In: PloS one. 2014 ; Vol. 9, No. 6.
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