Molecular analysis of suppression of interleukin-8 production by rebamipide in Helicobacter pylori-stimulated gastric cancer cell lines

M. Aihara, A. Azuma, H. Takizawa, Daisuke Tsuchimoto, Y. Funakoshi, Y. Shindo, Y. Ohmoto, K. Imagawa, M. Kikuchi, N. Mukaida, K. Matsushima

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Abstract

Interleukin-8 (IL-8) may play an important role in Helicobacter pylori infection-associated chronic active gastritis and peptic ulcer disease in human. We have recently reported that a gastric cancer cell line, MKN45, produced a massive amount of IL-8 upon coculture with live H. pylori. Moreover, H. pylori induced the activation of NF-κB as well as AP-1, leading to IL-8 gene transcription. In this study, we evaluated the effect of rebamipide, an antigastritis and antiulcer agent, on H. pylori-induced IL-8 production. Rebamipide inhibited the production of IL-8 in several gastric cancer cell lines infected with H. pylori. In addition, rebamipide suppressed H. pylori-induced IL-8 gene expression at the transcriptional level as revealed by northern blotting analysis and luciferase activity in cells that were transfected with a luciferase expression vector linked with a 5'- flanking region of the IL-8 gene (bp -133 to +44). Furthermore, rebamipide significantly suppressed the NF-κB activation by H. pylori infection. These results suggest that rebamipide may protect against the mucosal inflammation associated with H. pylori infection through inhibition of a proinflammatory cytokine, IL-8.

Original languageEnglish
JournalDigestive Diseases and Sciences
Volume43
Issue number9 SUPPL.
Publication statusPublished - Oct 3 1998
Externally publishedYes

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Interleukin-8
Helicobacter pylori
Stomach Neoplasms
Cell Line
Helicobacter Infections
Luciferases
rebamipide
5' Flanking Region
Transcription Factor AP-1
Gastritis
Coculture Techniques
Peptic Ulcer
Northern Blotting
Genes
Cytokines
Inflammation
Gene Expression

All Science Journal Classification (ASJC) codes

  • Physiology
  • Gastroenterology

Cite this

Molecular analysis of suppression of interleukin-8 production by rebamipide in Helicobacter pylori-stimulated gastric cancer cell lines. / Aihara, M.; Azuma, A.; Takizawa, H.; Tsuchimoto, Daisuke; Funakoshi, Y.; Shindo, Y.; Ohmoto, Y.; Imagawa, K.; Kikuchi, M.; Mukaida, N.; Matsushima, K.

In: Digestive Diseases and Sciences, Vol. 43, No. 9 SUPPL., 03.10.1998.

Research output: Contribution to journalArticle

Aihara, M, Azuma, A, Takizawa, H, Tsuchimoto, D, Funakoshi, Y, Shindo, Y, Ohmoto, Y, Imagawa, K, Kikuchi, M, Mukaida, N & Matsushima, K 1998, 'Molecular analysis of suppression of interleukin-8 production by rebamipide in Helicobacter pylori-stimulated gastric cancer cell lines', Digestive Diseases and Sciences, vol. 43, no. 9 SUPPL..
Aihara, M. ; Azuma, A. ; Takizawa, H. ; Tsuchimoto, Daisuke ; Funakoshi, Y. ; Shindo, Y. ; Ohmoto, Y. ; Imagawa, K. ; Kikuchi, M. ; Mukaida, N. ; Matsushima, K. / Molecular analysis of suppression of interleukin-8 production by rebamipide in Helicobacter pylori-stimulated gastric cancer cell lines. In: Digestive Diseases and Sciences. 1998 ; Vol. 43, No. 9 SUPPL.
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