Molecular and biochemical characterization of a Zeta-class glutathione S-transferase of the silkmoth

Kohji Yamamoto, Yuichi Shigeoka, Yoichi Aso, Yutaka Banno, Makoto Kimura, Takashi Nakashima

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

A cDNA encoding Zeta-class GST of the silkmoth, Bombyx mori (bmGSTZ), was cloned by a reverse transcriptase-polymerase chain reaction. The resulting clone was sequenced and deduced for amino acid sequence, which revealed 45-50% identities to Zeta-class GSTs from other organisms. A recombinant protein (rbmGSTZ) was functionally overexpressed in Bombyx mori cells in a soluble form and purified to homogeneity. rbmGSTZ was able to catalyze the biotranslation of glutathione with dichloroacetic acid. We found that the present GST-catalyzed dechlorination of permethrin and distributed abundantly in silkmoth strain exhibiting permethrin resistance. Our results suggest that bmGSTZ could contribute to permethrin resistance in lepidopteran.

Original languageEnglish
Pages (from-to)30-35
Number of pages6
JournalPesticide Biochemistry and Physiology
Volume94
Issue number1
DOIs
Publication statusPublished - May 1 2009

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Health, Toxicology and Mutagenesis

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