Molecular and biochemical characterization of a Zeta-class glutathione S-transferase of the silkmoth

Kohji Yamamoto, Yuichi Shigeoka, Yoichi Aso, Yutaka Banno, Makoto Kimura, Takashi Nakashima

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

A cDNA encoding Zeta-class GST of the silkmoth, Bombyx mori (bmGSTZ), was cloned by a reverse transcriptase-polymerase chain reaction. The resulting clone was sequenced and deduced for amino acid sequence, which revealed 45-50% identities to Zeta-class GSTs from other organisms. A recombinant protein (rbmGSTZ) was functionally overexpressed in Bombyx mori cells in a soluble form and purified to homogeneity. rbmGSTZ was able to catalyze the biotranslation of glutathione with dichloroacetic acid. We found that the present GST-catalyzed dechlorination of permethrin and distributed abundantly in silkmoth strain exhibiting permethrin resistance. Our results suggest that bmGSTZ could contribute to permethrin resistance in lepidopteran.

Original languageEnglish
Pages (from-to)30-35
Number of pages6
JournalPesticide Biochemistry and Physiology
Volume94
Issue number1
DOIs
Publication statusPublished - May 1 2009

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Bombyx
permethrin
Glutathione Transferase
Permethrin
glutathione transferase
Bombyx mori
dichloroacetic acid
dechlorination
Dichloroacetic Acid
recombinant proteins
glutathione
amino acid sequences
Reverse Transcriptase Polymerase Chain Reaction
reverse transcriptase polymerase chain reaction
Lepidoptera
Recombinant Proteins
clones
Glutathione
Amino Acid Sequence
Complementary DNA

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Health, Toxicology and Mutagenesis

Cite this

Molecular and biochemical characterization of a Zeta-class glutathione S-transferase of the silkmoth. / Yamamoto, Kohji; Shigeoka, Yuichi; Aso, Yoichi; Banno, Yutaka; Kimura, Makoto; Nakashima, Takashi.

In: Pesticide Biochemistry and Physiology, Vol. 94, No. 1, 01.05.2009, p. 30-35.

Research output: Contribution to journalArticle

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AU - Nakashima, Takashi

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