Molecular and biological characterization of Chrysanthemum stem necrosis virus isolates from distinct regions in Japan

Minoru Takeshita, Naoko Nagai, Mitsuru Okuda, Shohei Matsuura, Shiori Okuda, Naruto Furuya, Kenichi Tsuchiya

Research output: Contribution to journalArticle

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Abstract

Three isolates of Chrysanthemum stem necrosis virus (CSNV) were obtained from chrysanthemum plants in distinct regions of Japan in 2006 and 2007. All the original host plants showed severe necrotic symptoms on the leaves and stems. Amino acid sequence data of the nucleocapsid protein genes of the three isolates (CbCh07A, TcCh07A, and GnCh07S) showed high identities with those of two other CSNV isolates, HiCh06A L1 from Japan and Chry1 from Brazil. Furthermore, for the first time the complete nucleotide sequence of the S RNA was determined for CSNV (isolate HiCh06A). In phylogenetic analysis based on the non-structural protein genes from the genus Tospovirus, HiCh06A L1 was placed in the same genetic group as Tomato spotted wilt virus (TSWV) and Impatiens necrotic spot virus. Host range examination for isolates HiCh06A L1 and CbCh07A showed that green pepper (cv. 'Kyoyutaka', 'Saitamawase', 'Tosakatsura', 'L3 sarara' and 'L3 miogi') and tomato (cv. 'Sekaiichitomato') were systemically susceptible hosts, whereas TSWV-resistant Solanaceae species, Capsicum chinense, Lycopersicon peruvianum and a TSWV-resistant cultivar of green pepper (cv. TSR miogi), were resistant.

Original languageEnglish
Pages (from-to)9-14
Number of pages6
JournalEuropean Journal of Plant Pathology
Volume131
Issue number1
DOIs
Publication statusPublished - Sep 1 2011

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Chrysanthemum stem necrosis virus
Tomato spotted wilt virus
sweet peppers
Japan
Impatiens necrotic spot virus
Solanum peruvianum
Capsicum chinense
nucleocapsid proteins
Tospovirus
Chrysanthemum
Solanaceae
host range
signs and symptoms (plants)
genes
host plants
amino acid sequences
tomatoes
RNA
nucleotide sequences
Brazil

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science
  • Horticulture

Cite this

Molecular and biological characterization of Chrysanthemum stem necrosis virus isolates from distinct regions in Japan. / Takeshita, Minoru; Nagai, Naoko; Okuda, Mitsuru; Matsuura, Shohei; Okuda, Shiori; Furuya, Naruto; Tsuchiya, Kenichi.

In: European Journal of Plant Pathology, Vol. 131, No. 1, 01.09.2011, p. 9-14.

Research output: Contribution to journalArticle

Takeshita, Minoru ; Nagai, Naoko ; Okuda, Mitsuru ; Matsuura, Shohei ; Okuda, Shiori ; Furuya, Naruto ; Tsuchiya, Kenichi. / Molecular and biological characterization of Chrysanthemum stem necrosis virus isolates from distinct regions in Japan. In: European Journal of Plant Pathology. 2011 ; Vol. 131, No. 1. pp. 9-14.
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