Molecular and epidemiological study of the first outbreak of vanB type vancomycin-resistant Enterococcus faecalis in Japan

Kozue Oana, Yoshiyuki Kawakami, Makoto Ohnishi, Masayo Ishikawa, Masako Hirota, Minoru Tozuka, Kenichi Atarashi, Kousuke Baba, Kyoko Fujiki, Mitsuo Okazaki, Takayuki Honda, Tetsuya Hayashi

Research output: Contribution to journalArticle

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Abstract

In July, 1999, an outbreak of vancomycin-resistant Enterococcus faecalis (VREF) with the vanB genotype occurred for the first time in Japan at Hokushin General Hospital, Nakano City, Nagano Prefecture. Four VREF strains were isolated from the clinical specimens of four inpatients, and 16 VREF strains were isolated by the screening of asymptomatic carriers and by surveillance of the hospital environment. All of the isolates possessed vanB genes. In a pulsed-field gel electrophoresis analysis, 19 out of 20 VREF isolates exhibited the indistinguishable restriction endonuclease digestion patterns of the chromosomal DNA. Additional investigation by Southern hybridization using the vanB probe implied that the vanB gene of these 19 isolates was encoded on a 110-kb plasmid. These findings indicate that the outbreak was principally caused by a single clone. The restriction endonuclease digestion patterns of the remaining single isolate was different from those of the other isolates. The vanB gene was encoded on the chromosome.

Original languageEnglish
Pages (from-to)17-22
Number of pages6
JournalJapanese Journal of Infectious Diseases
Volume54
Issue number1
Publication statusPublished - Jun 13 2001
Externally publishedYes

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Enterococcus faecalis
Disease Outbreaks
Epidemiologic Studies
Japan
DNA Restriction Enzymes
Digestion
Genes
Pulsed Field Gel Electrophoresis
General Hospitals
Inpatients
Plasmids
Clone Cells
Chromosomes
Genotype
Vancomycin-Resistant Enterococci
DNA

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

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Molecular and epidemiological study of the first outbreak of vanB type vancomycin-resistant Enterococcus faecalis in Japan. / Oana, Kozue; Kawakami, Yoshiyuki; Ohnishi, Makoto; Ishikawa, Masayo; Hirota, Masako; Tozuka, Minoru; Atarashi, Kenichi; Baba, Kousuke; Fujiki, Kyoko; Okazaki, Mitsuo; Honda, Takayuki; Hayashi, Tetsuya.

In: Japanese Journal of Infectious Diseases, Vol. 54, No. 1, 13.06.2001, p. 17-22.

Research output: Contribution to journalArticle

Oana, K, Kawakami, Y, Ohnishi, M, Ishikawa, M, Hirota, M, Tozuka, M, Atarashi, K, Baba, K, Fujiki, K, Okazaki, M, Honda, T & Hayashi, T 2001, 'Molecular and epidemiological study of the first outbreak of vanB type vancomycin-resistant Enterococcus faecalis in Japan', Japanese Journal of Infectious Diseases, vol. 54, no. 1, pp. 17-22.
Oana, Kozue ; Kawakami, Yoshiyuki ; Ohnishi, Makoto ; Ishikawa, Masayo ; Hirota, Masako ; Tozuka, Minoru ; Atarashi, Kenichi ; Baba, Kousuke ; Fujiki, Kyoko ; Okazaki, Mitsuo ; Honda, Takayuki ; Hayashi, Tetsuya. / Molecular and epidemiological study of the first outbreak of vanB type vancomycin-resistant Enterococcus faecalis in Japan. In: Japanese Journal of Infectious Diseases. 2001 ; Vol. 54, No. 1. pp. 17-22.
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AU - Atarashi, Kenichi

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