Molecular and genetic analysis of multiple changes in the levels of production of virulence factors in a subcultured variant of Streptococcus mutans

Yoshihisa Yamashita, Yuichi Tsukioka, Yoshio Nakano, Yukie Shibata, Toshihiko Koga

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We previously isolated a variant strain, Xcl00L, which shows decreased production of a surface protein antigen with a molecular mass of 190 kDa (PAc), after repeated subculturing of Streptococcus mutans strain Xc [Koga, T. et al. (1989) J. Gen. Microbiol. 135, 3199-3202]. In the present study, the levels of expression of the gtfB, gtfC, gtfD and ftf genes coding for polysaccharide-synthesizing enzymes in strain Xc100L were compared with those in strain Xc. Western blot analysis revealed multiple differences in the levels of production of these enzymes between these two strains. The amounts of the gtfB and gtfC gene products responsible for water-insoluble glucan synthesis in strain Xc100L were lower than those in strain Xc, whereas the amounts of the gtfD and ftf gene products responsible for water-soluble glucan synthesis and fructan synthesis, respectively, in strain Xc100L were higher than those in strain Xc. Northern blot analysis revealed that the amounts of the four enzymes and PAc produced by strain Xc100L reflected the relative amounts of mRNAs from the genes. The chloramphenicol acetyltransferase gene was fused with each of these five genes, and the transcriptional activity of each gene in strain Xc100L was quantitatively compared with that in strain Xc. The chloramphenicol acetyltransferase assay also indicated that the phenotypic differences between strain Xc and strain Xc100L were due to differences in the transcriptional activities of the virulence genes. No differences in the nucleotide sequences of the promoter regions of the gtfB, gtfC, gtfD, ftf and pac genes were found between strain Xc and strain Xc100L. It is possible that a factor(s) affecting the levels of transcription of the multiple virulence genes exists in S. mutans.

Original languageEnglish
Pages (from-to)81-87
Number of pages7
JournalFEMS microbiology letters
Volume144
Issue number1
DOIs
Publication statusPublished - Oct 15 1996

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Streptococcus mutans
Virulence Factors
Molecular Biology
Genes
Chloramphenicol O-Acetyltransferase
Glucans
Virulence
Enzymes
Fructans
Water
Surface Antigens
Genetic Promoter Regions
Northern Blotting
Polysaccharides
Membrane Proteins
Western Blotting
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Molecular and genetic analysis of multiple changes in the levels of production of virulence factors in a subcultured variant of Streptococcus mutans. / Yamashita, Yoshihisa; Tsukioka, Yuichi; Nakano, Yoshio; Shibata, Yukie; Koga, Toshihiko.

In: FEMS microbiology letters, Vol. 144, No. 1, 15.10.1996, p. 81-87.

Research output: Contribution to journalArticle

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