Molecular bases for human complement C7 polymorphisms, C7*3 and C7*4

Takahiko Horiuchi, Hiroaki Nishimukai, Tatsuyuki Okiura, Koji Nishimura, Hiroaki Nishizaka, Takeshi Kojima, Hiroshi Tsukamoto, Kenshi Hayashi, Mine Harada

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Complement C7 is one of the components of membrane attack complex (MAC) generated by the terminal complement cascade. C7 protein is polymorphic and most of its polymorphisms have been identified using isoelectric focusing (IEF), which detects protein charge differences. To date, the molecular bases of the polymorphisms detected by IEF have not been determined. In this paper, we describe the structural bases of two C7 IEF-detected polymorphisms, C7*3 and C7*4, both of which are common in Asian populations. C7*3 resulted from substitution of cysteine (Cys) at amino acid residue 106 by charged arginine (Arg; C106R), while charged lysine (Lys) at amino acid residue 398 was replaced by neutral glutamine (Gln; K398Q) in C7*4. As C7*3 is hypomorphic, it is important to study its possible associations with diseases such as immunological disorders and infections. We present genetic bases for this C7 polymorphism, which we determined using polymerase chain reaction (PCR)-based genotyping, a simple and accurate method suitable for large-scale studies.

Original languageEnglish
Pages (from-to)450-455
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume298
Issue number3
DOIs
Publication statusPublished - Nov 25 2002

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Complement C7
Isoelectric Focusing
Polymorphism
Complement Membrane Attack Complex
Amino Acids
Glutamine
Lysine
Cysteine
Arginine
Polymerase chain reaction
Proteins
Polymerase Chain Reaction
Substitution reactions
Infection
Population

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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Molecular bases for human complement C7 polymorphisms, C7*3 and C7*4. / Horiuchi, Takahiko; Nishimukai, Hiroaki; Okiura, Tatsuyuki; Nishimura, Koji; Nishizaka, Hiroaki; Kojima, Takeshi; Tsukamoto, Hiroshi; Hayashi, Kenshi; Harada, Mine.

In: Biochemical and Biophysical Research Communications, Vol. 298, No. 3, 25.11.2002, p. 450-455.

Research output: Contribution to journalArticle

Horiuchi, T, Nishimukai, H, Okiura, T, Nishimura, K, Nishizaka, H, Kojima, T, Tsukamoto, H, Hayashi, K & Harada, M 2002, 'Molecular bases for human complement C7 polymorphisms, C7*3 and C7*4', Biochemical and Biophysical Research Communications, vol. 298, no. 3, pp. 450-455. https://doi.org/10.1016/S0006-291X(02)02481-6
Horiuchi, Takahiko ; Nishimukai, Hiroaki ; Okiura, Tatsuyuki ; Nishimura, Koji ; Nishizaka, Hiroaki ; Kojima, Takeshi ; Tsukamoto, Hiroshi ; Hayashi, Kenshi ; Harada, Mine. / Molecular bases for human complement C7 polymorphisms, C7*3 and C7*4. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 298, No. 3. pp. 450-455.
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