Molecular changes associated with adaptation of equine influenza H3N8 virus in embryonated chicken eggs

Awlad Wadair Ali Said, Michiko Kodani, Tatsufumi Usui, Yoshikazu Fujimoto, Toshihiro Ito, Tsuyoshi Yamaguchi

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Embryonated chicken eggs (ECEs) are routinely used to isolate equine influenza virus. Propagation of the virus in ECEs results in selection of variants. In the present study, we determined nucleotide sequences of entire coding regions of parent A/equine/ Tottori/1/07 (H3N8) and its derivatives that have different passage histories in ECE. After 12 passages, nucleotide sequence analysis predicted 3 amino acid substitutions in hemagglutinin (HA; 2 in HA1 and 1 in HA2). The two amino acid substitutions in HA1 were located in the vicinity of the cell receptor-binding site. Three other amino acid substitutions were predicted in internal proteins, 1 in the M1, 1 in the NP and 1 in the PA. This is the first report showing mutations in the internal protein genes of equine influenza virus associated with adaptation to ECE.

Original languageEnglish
Pages (from-to)545-548
Number of pages4
JournalJournal of Veterinary Medical Science
Issue number4
Publication statusPublished - Apr 1 2011


All Science Journal Classification (ASJC) codes

  • veterinary(all)

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