Molecular characterization and comparative study of 6 salivary gland metalloproteases from the hard tick, Haemaphysalis longicornis

Thasaneeya Harnnoi, Takeshi Sakaguchi, Yoshifumi Nishikawa, Xuenan Xuan, Kozo Fujisaki

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Six genes encoding metalloproteases were identified from the salivary gland of the hard tick, Haemaphysalis longicornis. Comparative analyses have shown the evolutionary distinct and different mRNA expression patterns of each gene during blood feeding. The proteins are synthesized as proenzymes with a prodomain and a metalloprotease/cysteine-rich domain of the reprolysin family. Within the active site, amino acid substitutions were observed. The recombinant Escherichia coli expression of one gene, hlESTMP1, was performed. The immunoblot analysis and indirect fluorescent assay using anti-hlESTMP1 suggested that this protein is mainly expressed in the cytoplasm of the salivary glands and only the mature form of 34 kDa was detectable. The proenzyme expressed by baculovirus was processed into a mature domain, suggesting that proenzyme activation possibly occurs through a pro-protein convertase dependent pathway. The presence of these diverse enzymes might contribute to the greater functional complexity of bioactive molecules in tick saliva to facilitate blood feeding.

Original languageEnglish
Pages (from-to)93-101
Number of pages9
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume147
Issue number1
DOIs
Publication statusPublished - May 1 2007

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Ixodidae
Enzyme Precursors
Metalloproteases
Salivary Glands
Blood
Genes
Proteins
Gene encoding
Baculoviridae
Ticks
Amino Acid Substitution
Saliva
Escherichia coli
Cysteine
Assays
Catalytic Domain
Cytoplasm
Substitution reactions
Chemical activation
Gene Expression

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology

Cite this

Molecular characterization and comparative study of 6 salivary gland metalloproteases from the hard tick, Haemaphysalis longicornis. / Harnnoi, Thasaneeya; Sakaguchi, Takeshi; Nishikawa, Yoshifumi; Xuan, Xuenan; Fujisaki, Kozo.

In: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, Vol. 147, No. 1, 01.05.2007, p. 93-101.

Research output: Contribution to journalArticle

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