TY - JOUR
T1 - Molecular characterization, localization, and distribution of innexins in the silkworm, bombyx mori
AU - Hong, Sun Mee
AU - Noh, Si Kab
AU - Kim, Kyung A.
AU - Mitsunobu, Hitoshi
AU - Mon, Hiroaki
AU - Lee, Jae Man
AU - Kawaguchi, Yutaka
AU - Kusakabe, Takahiro
N1 - Funding Information:
Acknowledgments This work was supported in part by the Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN) and by a grant from the Ministry of Agriculture, Forestry, and Fisheries of Japan (Integrated research project for plant, insect, and animal using genome technology INSECT-1201).
PY - 2009/9
Y1 - 2009/9
N2 - Gap junctions that allow for a direct exchange of second messenger and ions are the most conserved cellular structures in multicellular organisms. We have isolated and characterized a Bombyx mori gene innexin3 that encodes a new member of the innexin family required for the early embryonic development. The BmINX3 mRNA was 1,814 nucleotide residues in length, and the deduced amino acid sequence of BmInx3 shared 74% similarity with Apis melifera innexin3. The expression profile of the BmINX3 mRNA is similar to that of previously described BmINX2, expressed in ovary and testis after 5th instar larvae and in fat body after gut purge. However, during embryogenesis, the expression of BmINX3 mRNA is restricted to the blastokinesis stage. Microscopic observation of the BmInx2 and BmInx3 fused to fluorescent proteins showed an overlapping cytoplasmic expression, whereas the BmInx4 is accumulated in the cytoplasmic surface at which two cells have physical contact. This finding of innexins distribution in silkworm would provide an essential basis for future studies of the functions and interactions of innexins.
AB - Gap junctions that allow for a direct exchange of second messenger and ions are the most conserved cellular structures in multicellular organisms. We have isolated and characterized a Bombyx mori gene innexin3 that encodes a new member of the innexin family required for the early embryonic development. The BmINX3 mRNA was 1,814 nucleotide residues in length, and the deduced amino acid sequence of BmInx3 shared 74% similarity with Apis melifera innexin3. The expression profile of the BmINX3 mRNA is similar to that of previously described BmINX2, expressed in ovary and testis after 5th instar larvae and in fat body after gut purge. However, during embryogenesis, the expression of BmINX3 mRNA is restricted to the blastokinesis stage. Microscopic observation of the BmInx2 and BmInx3 fused to fluorescent proteins showed an overlapping cytoplasmic expression, whereas the BmInx4 is accumulated in the cytoplasmic surface at which two cells have physical contact. This finding of innexins distribution in silkworm would provide an essential basis for future studies of the functions and interactions of innexins.
UR - http://www.scopus.com/inward/record.url?scp=70350516835&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70350516835&partnerID=8YFLogxK
U2 - 10.1007/s12033-009-9175-7
DO - 10.1007/s12033-009-9175-7
M3 - Article
C2 - 19415533
AN - SCOPUS:70350516835
SN - 1073-6085
VL - 43
SP - 52
EP - 58
JO - Molecular Biotechnology
JF - Molecular Biotechnology
IS - 1
ER -