Molecular characterization, localization, and distribution of innexins in the silkworm, bombyx mori

Sun Mee Hong, Si Kab Noh, Kyung A. Kim, Hitoshi Mitsunobu, Hiroaki Mon, Man Lee, Yutaka Kawaguchi, Takahiro Kusakabe

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Gap junctions that allow for a direct exchange of second messenger and ions are the most conserved cellular structures in multicellular organisms. We have isolated and characterized a Bombyx mori gene innexin3 that encodes a new member of the innexin family required for the early embryonic development. The BmINX3 mRNA was 1,814 nucleotide residues in length, and the deduced amino acid sequence of BmInx3 shared 74% similarity with Apis melifera innexin3. The expression profile of the BmINX3 mRNA is similar to that of previously described BmINX2, expressed in ovary and testis after 5th instar larvae and in fat body after gut purge. However, during embryogenesis, the expression of BmINX3 mRNA is restricted to the blastokinesis stage. Microscopic observation of the BmInx2 and BmInx3 fused to fluorescent proteins showed an overlapping cytoplasmic expression, whereas the BmInx4 is accumulated in the cytoplasmic surface at which two cells have physical contact. This finding of innexins distribution in silkworm would provide an essential basis for future studies of the functions and interactions of innexins.

Original languageEnglish
Pages (from-to)52-58
Number of pages7
JournalMolecular Biotechnology
Volume43
Issue number1
DOIs
Publication statusPublished - Sep 1 2009

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Bombyx
Messenger RNA
Embryonic Development
Fat Body
Bees
Gap Junctions
Second Messenger Systems
Cellular Structures
Nucleotides
Oils and fats
Larva
Testis
Amino acids
Amino Acid Sequence
Ovary
Ion exchange
Genes
Fats
Ions
Proteins

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Cite this

Molecular characterization, localization, and distribution of innexins in the silkworm, bombyx mori. / Hong, Sun Mee; Noh, Si Kab; Kim, Kyung A.; Mitsunobu, Hitoshi; Mon, Hiroaki; Lee, Man; Kawaguchi, Yutaka; Kusakabe, Takahiro.

In: Molecular Biotechnology, Vol. 43, No. 1, 01.09.2009, p. 52-58.

Research output: Contribution to journalArticle

Hong, Sun Mee ; Noh, Si Kab ; Kim, Kyung A. ; Mitsunobu, Hitoshi ; Mon, Hiroaki ; Lee, Man ; Kawaguchi, Yutaka ; Kusakabe, Takahiro. / Molecular characterization, localization, and distribution of innexins in the silkworm, bombyx mori. In: Molecular Biotechnology. 2009 ; Vol. 43, No. 1. pp. 52-58.
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