Molecular characterization of a new begomovirus infecting honeysuckle in Kobe, Japan

Hassan Karakacha Were, Minoru Takeshita, Naruto Furuya, Yoichi Takanami

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A virus (HY12) isolated from honeysuckle (Lonicera japonica Thunb.) collected in Kobe, Hyogo prefecture, Japan, showing symptoms of yellow vein mosaic, was characterized. HY12 was transmitted to Nicotiana benthamiana by sap inoculation while the other honeysuckle isolates could not. The complete DNA-A and DNA-β like molecules of the isolate were determined and found to comprise 2763 and 1262 nucleotides (nt), respectively, with a typical begomovirus genome organization. The DNA β molecule had the conserved ORF (β Cl) and satellite conserved region (SCR) typical of the Geminiviridae family containing a 5′-TAATATTAC-3′ motif in the stem loop and the adenine-(A) rich region. Additionally, its entire sequence was interrupted at intervals with sequences that were not viral and whose origin was unknown. A comparison of the DNA-A sequences revealed that HY12 shared 74% with four other honeysuckle begomoviruses but 88-89% with tobacco begomoviruses found in Japan. The isolate shared up to 92% of the IR sequences with the honeysuckle begomoviruses but only 60-88% with those of tobacco. Phylogenetic analysis of full length DNA-A component as well as its CP clustered HY12 with the tobacco begomoviruses. HY12 is an isolate of Honeysuckle yellow vein mosaic virus.

Original languageEnglish
Pages (from-to)61-71
Number of pages11
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume50
Issue number1
Publication statusPublished - Feb 1 2005

Fingerprint

Begomovirus
Lonicera
A-DNA
Japan
Tobacco
tobacco
Honeysuckle yellow vein mosaic virus
DNA
Lonicera japonica
Veins
Geminiviridae
Nicotiana benthamiana
Mosaic Viruses
plant veins
adenine
sap
signs and symptoms (plants)
Adenine
open reading frames
Open Reading Frames

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

Molecular characterization of a new begomovirus infecting honeysuckle in Kobe, Japan. / Were, Hassan Karakacha; Takeshita, Minoru; Furuya, Naruto; Takanami, Yoichi.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 50, No. 1, 01.02.2005, p. 61-71.

Research output: Contribution to journalArticle

Were, Hassan Karakacha ; Takeshita, Minoru ; Furuya, Naruto ; Takanami, Yoichi. / Molecular characterization of a new begomovirus infecting honeysuckle in Kobe, Japan. In: Journal of the Faculty of Agriculture, Kyushu University. 2005 ; Vol. 50, No. 1. pp. 61-71.
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abstract = "A virus (HY12) isolated from honeysuckle (Lonicera japonica Thunb.) collected in Kobe, Hyogo prefecture, Japan, showing symptoms of yellow vein mosaic, was characterized. HY12 was transmitted to Nicotiana benthamiana by sap inoculation while the other honeysuckle isolates could not. The complete DNA-A and DNA-β like molecules of the isolate were determined and found to comprise 2763 and 1262 nucleotides (nt), respectively, with a typical begomovirus genome organization. The DNA β molecule had the conserved ORF (β Cl) and satellite conserved region (SCR) typical of the Geminiviridae family containing a 5′-TAATATTAC-3′ motif in the stem loop and the adenine-(A) rich region. Additionally, its entire sequence was interrupted at intervals with sequences that were not viral and whose origin was unknown. A comparison of the DNA-A sequences revealed that HY12 shared 74{\%} with four other honeysuckle begomoviruses but 88-89{\%} with tobacco begomoviruses found in Japan. The isolate shared up to 92{\%} of the IR sequences with the honeysuckle begomoviruses but only 60-88{\%} with those of tobacco. Phylogenetic analysis of full length DNA-A component as well as its CP clustered HY12 with the tobacco begomoviruses. HY12 is an isolate of Honeysuckle yellow vein mosaic virus.",
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