Molecular characterization of a new begomovirus infecting tobacco in Kagoshima, Japan

An evidence for interspecific recombination

Hassan Karakacha Were, Minoru Takeshita, Naruto Furuya, Yoichi Takanami

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A virus infected tobacco sample (KG5Tob) showing leaf curl symptoms was collected in Kagoshima, Japan. The complete DNA-A like molecule of KG5Tob was determined and found to comprise 2762 nucleotides with a typical begomovirus genome organization. A comparison of the DNA-A sequences revealed that KG5Tob shared 87% with its closest relatives, Honeysuckle yellow vein mosaic virus (HYVMV) and Tobacco leaf curl Yamaguchi virus (TbLCYV), however, on comparison of the intergenic region (IR) nt sequences, the isolate shared only 66 and 64% of the IR sequences with the former and the latter, respectively. Phylogenetic analysis of full length DNA-A component clustered KG5Tob in a lone cluster but close to HYVMV and TbLCYV. When the CPs were aligned, similar results were observed and KG5Tob was placed in a lone cluster. A comparison of the Other ORFs showed that different parts of the genome had different affinities, suggesting that recombination could have played a major role in the evolution of this isolate. Molecular data show that KG5Tob isolate is an isolate of HYVMV, although the isolate was obtained from tobacco.

Original languageEnglish
Pages (from-to)83-91
Number of pages9
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume50
Issue number1
Publication statusPublished - Feb 1 2005

Fingerprint

Honeysuckle yellow vein mosaic virus
Begomovirus
Lonicera
A-DNA
Horizontal Gene Transfer
Intergenic DNA
Tobacco leaf curl Japan virus
Tobacco
Tobacco Mosaic Virus
Veins
Japan
tobacco
Viruses
intergenic DNA
Genome
Mosaic Viruses
leaf curling
genome
DNA
Genetic Recombination

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

Molecular characterization of a new begomovirus infecting tobacco in Kagoshima, Japan : An evidence for interspecific recombination. / Were, Hassan Karakacha; Takeshita, Minoru; Furuya, Naruto; Takanami, Yoichi.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 50, No. 1, 01.02.2005, p. 83-91.

Research output: Contribution to journalArticle

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