Molecular characterization of a novel β-glucuronidase from Scutellaria baicalensis Georgi

K. Sasaki, F. Taura, Y. Shoyama, S. Morimoto

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We cloned a gene encoding Scutellaria β-glucuronidase (sGUS) that is involved in the initiation of H2O2 metabolism in skullcap (Scutellaria baicalensis). This gene consists of a 1581-nucleotide open reading frame, the deduced amino acid sequence of which contains an ATP/GTP binding site and a leucine zipper motif. sGUS has apparent similarity to the heparan sulfate-metabolizing β-glucuronidase heparanase but no homology to family 2 β-glucuronidases. In addition, neither the family 2 glycosylhydrolase signature nor family 2 acid-base catalyst was found in this enzyme. These results suggested that sGUS does not belong to the family 2 β-glucuronidases. We modified several residues predicted to act as the acid-base or nucleophilic residue of sGUS by site-directed mutagenesis. Mutations at Glu212 or Glu329 resulted in much lower k(cat)/K(m) values in the mutants as compared with the wild-type enzyme, indicating that these are the acid-base and nucleophilic residues of the active site, respectively. Moreover, similar site-directed mutagenesis confirmed that Tyr281 is also involved in the β-glucuronidase activity. The amino acid sequences of small regions containing these active site residues were conserved in heparanases. As sGUS has various structural characteristics in common with heparanase, we concluded that sGUS and heparanase belong to the same new family.

Original languageEnglish
Pages (from-to)27466-27472
Number of pages7
JournalJournal of Biological Chemistry
Volume275
Issue number35
DOIs
Publication statusPublished - Sep 1 2000

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Scutellaria baicalensis
Glucuronidase
Scutellaria
Mutagenesis
Site-Directed Mutagenesis
Acids
Amino Acid Sequence
Catalytic Domain
Amino Acids
Leucine Zippers
Gene encoding
Heparitin Sulfate
Enzymes
Guanosine Triphosphate
Metabolism
Open Reading Frames
Genes
Nucleotides
Adenosine Triphosphate
Binding Sites

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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Molecular characterization of a novel β-glucuronidase from Scutellaria baicalensis Georgi. / Sasaki, K.; Taura, F.; Shoyama, Y.; Morimoto, S.

In: Journal of Biological Chemistry, Vol. 275, No. 35, 01.09.2000, p. 27466-27472.

Research output: Contribution to journalArticle

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