Molecular characterization of fungal populations on the tongue dorsum of institutionalized elderly adults

H. Li, T. Takeshita, M. Furuta, M. Tomioka, Y. Shibata, Y. Shimazaki, K. Makimura, Y. Yamashita

Research output: Contribution to journalArticle

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Abstract

Objectives: To characterize the global composition of oral fungal populations in frail elderly adults and to investigate the relationship with their health status. Materials and methods: We investigated the fungal populations on the tongue dorsum in 291 institutionalized elderly adults by molecular PCR-based techniques using internal transcribed spacer regions of nuclear ribosomal DNA. Results: Quantitative PCR analysis showed that fungi were present on the tongue dorsum of 128 subjects at ≥104CFU per sample, and 35 of them exceeded 105CFU per sample. Length heterogeneity-PCR analysis and nucleotide sequence determinations showed that Candida albicans was most frequently detected in those subjects with fungi at ≥104CFU per sample (105 subjects), followed by Candida dubliniensis (78), Malassezia restricta (57), and Candida tropicalis (45). Statistical analysis revealed that those subjects with ≥105CFU of fungi other than C. albicans per sample had an increased risk of fever (≥7 febrile days per 12months) compared with subjects with <105CFU per sample, after adjustment for other fever-associated confounding factors. Conclusions: These data demonstrate that the oral cavity of the elderly is inhabited by a diverse array of fungi not limited to typical Candida species and they suggest that the diversity in distribution is associated with health status.

Original languageEnglish
Pages (from-to)771-777
Number of pages7
JournalOral Diseases
Volume18
Issue number8
DOIs
Publication statusPublished - Nov 1 2012

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Tongue
Fungi
Fever
Candida albicans
Candida
Polymerase Chain Reaction
Population
Health Status
Malassezia
Candida tropicalis
Frail Elderly
Ribosomal DNA
Sequence Analysis
Mouth

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Dentistry(all)

Cite this

Molecular characterization of fungal populations on the tongue dorsum of institutionalized elderly adults. / Li, H.; Takeshita, T.; Furuta, M.; Tomioka, M.; Shibata, Y.; Shimazaki, Y.; Makimura, K.; Yamashita, Y.

In: Oral Diseases, Vol. 18, No. 8, 01.11.2012, p. 771-777.

Research output: Contribution to journalArticle

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AU - Furuta, M.

AU - Tomioka, M.

AU - Shibata, Y.

AU - Shimazaki, Y.

AU - Makimura, K.

AU - Yamashita, Y.

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