Molecular characterization of Ralstonia solanacearum strains causing bacterial wilt of solanaceous crops in Myanmar by rep-PCR analysis

Htet Wai Wai Kyaw, Kenichi Tsuchiya, Matsumoto Masaru, Seint San Aye, Kazuhiro Iiyama, Daisuke Kurose, Mitsuo Horita, Naruto Furuya

Research output: Contribution to journalArticle

Abstract

Seventy strains of Ralstonia solanacearum from solanaceous crops in Myanmar were molecularly characterized by rep-PCR DNA fingerprinting. Cluster analysis based on the fingerprints (total 33 fingerprint types) revealed seven main clusters at 80% similarity level. Strains in phylotype I showed 29 fingerprint types and were divided into six clusters, most of which were closely correlated with host plant, geographic origin, sequevar and/or biovar. In contrast, phylotype II strains showed four DNA fingerprint types within a single cluster. These results suggest that genetic diversity of the strains is endemic and that phylotype I and II may be invasive strains.

Original languageEnglish
Pages (from-to)33-38
Number of pages6
JournalJournal of General Plant Pathology
Volume85
Issue number1
DOIs
Publication statusPublished - Jan 25 2019

Fingerprint

bacterial wilt
Ralstonia solanacearum
Myanmar
crops
DNA fingerprinting
provenance
cluster analysis
host plants
genetic variation
phylotype

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

Molecular characterization of Ralstonia solanacearum strains causing bacterial wilt of solanaceous crops in Myanmar by rep-PCR analysis. / Kyaw, Htet Wai Wai; Tsuchiya, Kenichi; Masaru, Matsumoto; Aye, Seint San; Iiyama, Kazuhiro; Kurose, Daisuke; Horita, Mitsuo; Furuya, Naruto.

In: Journal of General Plant Pathology, Vol. 85, No. 1, 25.01.2019, p. 33-38.

Research output: Contribution to journalArticle

@article{1856c51a18c34ba1bb91b522740cf9f6,
title = "Molecular characterization of Ralstonia solanacearum strains causing bacterial wilt of solanaceous crops in Myanmar by rep-PCR analysis",
abstract = "Seventy strains of Ralstonia solanacearum from solanaceous crops in Myanmar were molecularly characterized by rep-PCR DNA fingerprinting. Cluster analysis based on the fingerprints (total 33 fingerprint types) revealed seven main clusters at 80{\%} similarity level. Strains in phylotype I showed 29 fingerprint types and were divided into six clusters, most of which were closely correlated with host plant, geographic origin, sequevar and/or biovar. In contrast, phylotype II strains showed four DNA fingerprint types within a single cluster. These results suggest that genetic diversity of the strains is endemic and that phylotype I and II may be invasive strains.",
author = "Kyaw, {Htet Wai Wai} and Kenichi Tsuchiya and Matsumoto Masaru and Aye, {Seint San} and Kazuhiro Iiyama and Daisuke Kurose and Mitsuo Horita and Naruto Furuya",
year = "2019",
month = "1",
day = "25",
doi = "10.1007/s10327-018-0818-z",
language = "English",
volume = "85",
pages = "33--38",
journal = "Journal of General Plant Pathology",
issn = "1345-2630",
publisher = "Springer Japan",
number = "1",

}

TY - JOUR

T1 - Molecular characterization of Ralstonia solanacearum strains causing bacterial wilt of solanaceous crops in Myanmar by rep-PCR analysis

AU - Kyaw, Htet Wai Wai

AU - Tsuchiya, Kenichi

AU - Masaru, Matsumoto

AU - Aye, Seint San

AU - Iiyama, Kazuhiro

AU - Kurose, Daisuke

AU - Horita, Mitsuo

AU - Furuya, Naruto

PY - 2019/1/25

Y1 - 2019/1/25

N2 - Seventy strains of Ralstonia solanacearum from solanaceous crops in Myanmar were molecularly characterized by rep-PCR DNA fingerprinting. Cluster analysis based on the fingerprints (total 33 fingerprint types) revealed seven main clusters at 80% similarity level. Strains in phylotype I showed 29 fingerprint types and were divided into six clusters, most of which were closely correlated with host plant, geographic origin, sequevar and/or biovar. In contrast, phylotype II strains showed four DNA fingerprint types within a single cluster. These results suggest that genetic diversity of the strains is endemic and that phylotype I and II may be invasive strains.

AB - Seventy strains of Ralstonia solanacearum from solanaceous crops in Myanmar were molecularly characterized by rep-PCR DNA fingerprinting. Cluster analysis based on the fingerprints (total 33 fingerprint types) revealed seven main clusters at 80% similarity level. Strains in phylotype I showed 29 fingerprint types and were divided into six clusters, most of which were closely correlated with host plant, geographic origin, sequevar and/or biovar. In contrast, phylotype II strains showed four DNA fingerprint types within a single cluster. These results suggest that genetic diversity of the strains is endemic and that phylotype I and II may be invasive strains.

UR - http://www.scopus.com/inward/record.url?scp=85053453808&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85053453808&partnerID=8YFLogxK

U2 - 10.1007/s10327-018-0818-z

DO - 10.1007/s10327-018-0818-z

M3 - Article

AN - SCOPUS:85053453808

VL - 85

SP - 33

EP - 38

JO - Journal of General Plant Pathology

JF - Journal of General Plant Pathology

SN - 1345-2630

IS - 1

ER -