Molecular characterization of Staphylococcus warneri catalase

Daisuke Fukuda, Kouhei Mizuno, Mamiko Kohno, Kenji Sonomoto, Ayaaki Ishizaki

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The catalase gene was cloned by screening a genomic DNA library of S. warneri ISK-1 strain with a strong catalase activity for complementation of the activity in catalase-deficient E. coli strain. Nucleotide sequence analysis of a 2.2-kb DNA fragment revealed an open reading frame, called katA, encoding a peptide of 504 amino acids with a calculated molecular mass of 58kDa. The predicted amino acid sequence showed high similarities with the monofunctional catalases. No similarities were found between katA product and catalase-peroxidase type enzymes. Electrophoretic mobility of katA product was close to that of the previously purified ISK-1 catalase. Catalase activity was lost when the 135 amino acids were deleted from the C-terminal region.

Original languageEnglish
Pages (from-to)213-223
Number of pages11
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume45
Issue number1
Publication statusPublished - Nov 1 2000

Fingerprint

Staphylococcus warneri
Staphylococcus
Catalase
catalase
Amino Acids
amino acids
Genomic Library
DNA libraries
Gene Library
Peroxidase
Open Reading Frames
Sequence Analysis
open reading frames
Amino Acid Sequence
peroxidase
amino acid sequences
sequence analysis
peptides
molecular weight
Escherichia coli

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

Fukuda, D., Mizuno, K., Kohno, M., Sonomoto, K., & Ishizaki, A. (2000). Molecular characterization of Staphylococcus warneri catalase. Journal of the Faculty of Agriculture, Kyushu University, 45(1), 213-223.

Molecular characterization of Staphylococcus warneri catalase. / Fukuda, Daisuke; Mizuno, Kouhei; Kohno, Mamiko; Sonomoto, Kenji; Ishizaki, Ayaaki.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 45, No. 1, 01.11.2000, p. 213-223.

Research output: Contribution to journalArticle

Fukuda, D, Mizuno, K, Kohno, M, Sonomoto, K & Ishizaki, A 2000, 'Molecular characterization of Staphylococcus warneri catalase', Journal of the Faculty of Agriculture, Kyushu University, vol. 45, no. 1, pp. 213-223.
Fukuda D, Mizuno K, Kohno M, Sonomoto K, Ishizaki A. Molecular characterization of Staphylococcus warneri catalase. Journal of the Faculty of Agriculture, Kyushu University. 2000 Nov 1;45(1):213-223.
Fukuda, Daisuke ; Mizuno, Kouhei ; Kohno, Mamiko ; Sonomoto, Kenji ; Ishizaki, Ayaaki. / Molecular characterization of Staphylococcus warneri catalase. In: Journal of the Faculty of Agriculture, Kyushu University. 2000 ; Vol. 45, No. 1. pp. 213-223.
@article{d73591358b464cd6b5eb89aedf64f047,
title = "Molecular characterization of Staphylococcus warneri catalase",
abstract = "The catalase gene was cloned by screening a genomic DNA library of S. warneri ISK-1 strain with a strong catalase activity for complementation of the activity in catalase-deficient E. coli strain. Nucleotide sequence analysis of a 2.2-kb DNA fragment revealed an open reading frame, called katA, encoding a peptide of 504 amino acids with a calculated molecular mass of 58kDa. The predicted amino acid sequence showed high similarities with the monofunctional catalases. No similarities were found between katA product and catalase-peroxidase type enzymes. Electrophoretic mobility of katA product was close to that of the previously purified ISK-1 catalase. Catalase activity was lost when the 135 amino acids were deleted from the C-terminal region.",
author = "Daisuke Fukuda and Kouhei Mizuno and Mamiko Kohno and Kenji Sonomoto and Ayaaki Ishizaki",
year = "2000",
month = "11",
day = "1",
language = "English",
volume = "45",
pages = "213--223",
journal = "Journal of the Faculty of Agriculture, Kyushu University",
issn = "0023-6152",
publisher = "Faculty of Agriculture, Kyushu University",
number = "1",

}

TY - JOUR

T1 - Molecular characterization of Staphylococcus warneri catalase

AU - Fukuda, Daisuke

AU - Mizuno, Kouhei

AU - Kohno, Mamiko

AU - Sonomoto, Kenji

AU - Ishizaki, Ayaaki

PY - 2000/11/1

Y1 - 2000/11/1

N2 - The catalase gene was cloned by screening a genomic DNA library of S. warneri ISK-1 strain with a strong catalase activity for complementation of the activity in catalase-deficient E. coli strain. Nucleotide sequence analysis of a 2.2-kb DNA fragment revealed an open reading frame, called katA, encoding a peptide of 504 amino acids with a calculated molecular mass of 58kDa. The predicted amino acid sequence showed high similarities with the monofunctional catalases. No similarities were found between katA product and catalase-peroxidase type enzymes. Electrophoretic mobility of katA product was close to that of the previously purified ISK-1 catalase. Catalase activity was lost when the 135 amino acids were deleted from the C-terminal region.

AB - The catalase gene was cloned by screening a genomic DNA library of S. warneri ISK-1 strain with a strong catalase activity for complementation of the activity in catalase-deficient E. coli strain. Nucleotide sequence analysis of a 2.2-kb DNA fragment revealed an open reading frame, called katA, encoding a peptide of 504 amino acids with a calculated molecular mass of 58kDa. The predicted amino acid sequence showed high similarities with the monofunctional catalases. No similarities were found between katA product and catalase-peroxidase type enzymes. Electrophoretic mobility of katA product was close to that of the previously purified ISK-1 catalase. Catalase activity was lost when the 135 amino acids were deleted from the C-terminal region.

UR - http://www.scopus.com/inward/record.url?scp=0034562575&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034562575&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0034562575

VL - 45

SP - 213

EP - 223

JO - Journal of the Faculty of Agriculture, Kyushu University

JF - Journal of the Faculty of Agriculture, Kyushu University

SN - 0023-6152

IS - 1

ER -