Molecular cloning and characterization of type Ш polyketide synthase from Plumbago zeylanica

Seiichi Sakamoto, Yui Morita, Gorawit Yusakul, Waraporn Putalun, Hiroyuki Tanaka, Satoshi Morimoto

Research output: Contribution to journalArticle

Abstract

Two cDNAs encoding type Ш polyketide synthase (PKS1) and chalcone synthase (CHS, PKS2), were cloned from fresh leaves of Plumbago zeylanica L. (P. zeylanica). Their heterologous expression revealed that PKS1 catalyzed the formation of five α-pyrones from three to six acetate units by accepting acetyl-CoA and malonyl-CoA. In contrast, PKS2 catalyzed the formation of naringenin and bisnoryangonin by accepting p-coumaroyl-CoA and malonyl-CoA. Naringenin is thought to be involved in the biosynthesis of various bioactive flavonoids. PKS2 can be used to molecular breeding to enhance the production of these useful secondary metabolites via its overexpression. (Figure presented.).

Original languageEnglish
JournalJournal of Asian Natural Products Research
DOIs
Publication statusAccepted/In press - 2020

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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