TY - JOUR
T1 - Molecular cloning and characterization of Xenopus RGS5
AU - Saitoh, Osamu
AU - Odagiri, Megumi
AU - Masuho, Ikuo
AU - Nomoto, Satoshi
AU - Kinoshita, Noriyuki
N1 - Funding Information:
We are grateful to Dr. Y. Yaoita for Xenopus cDNA library. We thank Dr. H. Nakata for helpful discussion. This work is supported by research grants from the Ministry of Education, Science, Sports and Culture of Japan (to N.K. and O.S.), from the Naito Foundation (to O.S.), and from the Brain Science Foundation (to O.S.).
PY - 2000/4/2
Y1 - 2000/4/2
N2 - We identified six genes that encode putative RGS proteins (XRGSI-VI) in developing Xenopus embryos using PCR amplification with degenerate primers corresponding to the conserved region (RGS domain) of known RGS proteins. RT-PCR analysis revealed that mRNAs of these XRGSs are differentially expressed during embryogenesis. At stage 1, only XRGSII mRNA was detected. On the other hand, expression of XRGSVI mRNA increased apparently at stage 14 and expression of three of other XRGS (III, IV,V) elevated between stage 25 and 40. To further characterize XRGS proteins expressed in Xenopus embryos, we isolated a cDNA clone for XRGSIII. Based on determined nucleotide sequence, XRGSIII was considered as a Xenopus homologue of mammalian RGS5 (XRGS5). Genetic analysis using the pheromone response halo assay showed that expression of XRGS5 inhibits yeast response to α-factor, suggesting that XRGS5 negatively regulates the G-protein-mediated signaling pathway in developing Xenopus embryos. (C) 2000 Academic Press.
AB - We identified six genes that encode putative RGS proteins (XRGSI-VI) in developing Xenopus embryos using PCR amplification with degenerate primers corresponding to the conserved region (RGS domain) of known RGS proteins. RT-PCR analysis revealed that mRNAs of these XRGSs are differentially expressed during embryogenesis. At stage 1, only XRGSII mRNA was detected. On the other hand, expression of XRGSVI mRNA increased apparently at stage 14 and expression of three of other XRGS (III, IV,V) elevated between stage 25 and 40. To further characterize XRGS proteins expressed in Xenopus embryos, we isolated a cDNA clone for XRGSIII. Based on determined nucleotide sequence, XRGSIII was considered as a Xenopus homologue of mammalian RGS5 (XRGS5). Genetic analysis using the pheromone response halo assay showed that expression of XRGS5 inhibits yeast response to α-factor, suggesting that XRGS5 negatively regulates the G-protein-mediated signaling pathway in developing Xenopus embryos. (C) 2000 Academic Press.
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U2 - 10.1006/bbrc.2000.2379
DO - 10.1006/bbrc.2000.2379
M3 - Article
C2 - 10733901
AN - SCOPUS:0034595105
SN - 0006-291X
VL - 270
SP - 34
EP - 39
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -