Molecular cloning and chromosomal assignment of the mouse C-Type natriuretic peptide (CNP) gene (NPPC)

Comparison with the human CNP gene (NPPC)

Yoshihiro Ogawa, Hiroshi Itoh, Yuka Yoshitake, Miho Inoue, Takaaki Yoshimasa, Tadao Serikawa, Kazuwa Nakao

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

The mouse C-type natriuretic peptide (CNP) genomic fragment was isolated from a mouse genomic DNA library. The mouse CNP gene is composed of at least two exons and one intron. The 5′-flanking region contains an array of cis -acting regulatory elements and a dinucleotide CA repeat (microsatellite). Analysis of the deduced amino acid sequences revealed that mouse preproCNP is a 126-amino-acid peptide and that its C-terminal 22-residue peptide preceded by Lys-Lys is identical to porcine, rat, and human CNPs. On the basis of the polymerase chain reaction-analyzed microsatellite length polymorphisms among recombinant inbred strains of mice, the CNP gene (Nppc) was assigned to mouse chromosome 1. Furthermore, the human CNP 5′-flanking region was extended for sequencing, and comparison of the mouse and human CNP genomic sequences revealed regions of conservation and diversity. Using somatic hybrid cell methodology, the CNP gene (NPPC) was assigned to human chromosome 2. The present study has added another locus to the conserved syntenic group in mice and humans.

Original languageEnglish
Pages (from-to)383-387
Number of pages5
JournalGenomics
Volume24
Issue number2
DOIs
Publication statusPublished - Nov 15 1994
Externally publishedYes

Fingerprint

C-Type Natriuretic Peptide
Molecular Cloning
Genes
5' Flanking Region
Microsatellite Repeats
lysyllysine
Dinucleotide Repeats
Inbred Strains Mice
Peptide Fragments
Chromosomes, Human, Pair 2
Genomic Library
Hybrid Cells
Chromosomes, Human, Pair 1
Protein Sequence Analysis
Human Chromosomes
4-nitrophenylphosphorylcholine
Gene Library
Introns
Exons
Swine

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Molecular cloning and chromosomal assignment of the mouse C-Type natriuretic peptide (CNP) gene (NPPC) : Comparison with the human CNP gene (NPPC). / Ogawa, Yoshihiro; Itoh, Hiroshi; Yoshitake, Yuka; Inoue, Miho; Yoshimasa, Takaaki; Serikawa, Tadao; Nakao, Kazuwa.

In: Genomics, Vol. 24, No. 2, 15.11.1994, p. 383-387.

Research output: Contribution to journalArticle

Ogawa, Yoshihiro ; Itoh, Hiroshi ; Yoshitake, Yuka ; Inoue, Miho ; Yoshimasa, Takaaki ; Serikawa, Tadao ; Nakao, Kazuwa. / Molecular cloning and chromosomal assignment of the mouse C-Type natriuretic peptide (CNP) gene (NPPC) : Comparison with the human CNP gene (NPPC). In: Genomics. 1994 ; Vol. 24, No. 2. pp. 383-387.
@article{20c8ee3c2b2d47839d5e2e60599b2fe6,
title = "Molecular cloning and chromosomal assignment of the mouse C-Type natriuretic peptide (CNP) gene (NPPC): Comparison with the human CNP gene (NPPC)",
abstract = "The mouse C-type natriuretic peptide (CNP) genomic fragment was isolated from a mouse genomic DNA library. The mouse CNP gene is composed of at least two exons and one intron. The 5′-flanking region contains an array of cis -acting regulatory elements and a dinucleotide CA repeat (microsatellite). Analysis of the deduced amino acid sequences revealed that mouse preproCNP is a 126-amino-acid peptide and that its C-terminal 22-residue peptide preceded by Lys-Lys is identical to porcine, rat, and human CNPs. On the basis of the polymerase chain reaction-analyzed microsatellite length polymorphisms among recombinant inbred strains of mice, the CNP gene (Nppc) was assigned to mouse chromosome 1. Furthermore, the human CNP 5′-flanking region was extended for sequencing, and comparison of the mouse and human CNP genomic sequences revealed regions of conservation and diversity. Using somatic hybrid cell methodology, the CNP gene (NPPC) was assigned to human chromosome 2. The present study has added another locus to the conserved syntenic group in mice and humans.",
author = "Yoshihiro Ogawa and Hiroshi Itoh and Yuka Yoshitake and Miho Inoue and Takaaki Yoshimasa and Tadao Serikawa and Kazuwa Nakao",
year = "1994",
month = "11",
day = "15",
doi = "10.1006/geno.1994.1633",
language = "English",
volume = "24",
pages = "383--387",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Molecular cloning and chromosomal assignment of the mouse C-Type natriuretic peptide (CNP) gene (NPPC)

T2 - Comparison with the human CNP gene (NPPC)

AU - Ogawa, Yoshihiro

AU - Itoh, Hiroshi

AU - Yoshitake, Yuka

AU - Inoue, Miho

AU - Yoshimasa, Takaaki

AU - Serikawa, Tadao

AU - Nakao, Kazuwa

PY - 1994/11/15

Y1 - 1994/11/15

N2 - The mouse C-type natriuretic peptide (CNP) genomic fragment was isolated from a mouse genomic DNA library. The mouse CNP gene is composed of at least two exons and one intron. The 5′-flanking region contains an array of cis -acting regulatory elements and a dinucleotide CA repeat (microsatellite). Analysis of the deduced amino acid sequences revealed that mouse preproCNP is a 126-amino-acid peptide and that its C-terminal 22-residue peptide preceded by Lys-Lys is identical to porcine, rat, and human CNPs. On the basis of the polymerase chain reaction-analyzed microsatellite length polymorphisms among recombinant inbred strains of mice, the CNP gene (Nppc) was assigned to mouse chromosome 1. Furthermore, the human CNP 5′-flanking region was extended for sequencing, and comparison of the mouse and human CNP genomic sequences revealed regions of conservation and diversity. Using somatic hybrid cell methodology, the CNP gene (NPPC) was assigned to human chromosome 2. The present study has added another locus to the conserved syntenic group in mice and humans.

AB - The mouse C-type natriuretic peptide (CNP) genomic fragment was isolated from a mouse genomic DNA library. The mouse CNP gene is composed of at least two exons and one intron. The 5′-flanking region contains an array of cis -acting regulatory elements and a dinucleotide CA repeat (microsatellite). Analysis of the deduced amino acid sequences revealed that mouse preproCNP is a 126-amino-acid peptide and that its C-terminal 22-residue peptide preceded by Lys-Lys is identical to porcine, rat, and human CNPs. On the basis of the polymerase chain reaction-analyzed microsatellite length polymorphisms among recombinant inbred strains of mice, the CNP gene (Nppc) was assigned to mouse chromosome 1. Furthermore, the human CNP 5′-flanking region was extended for sequencing, and comparison of the mouse and human CNP genomic sequences revealed regions of conservation and diversity. Using somatic hybrid cell methodology, the CNP gene (NPPC) was assigned to human chromosome 2. The present study has added another locus to the conserved syntenic group in mice and humans.

UR - http://www.scopus.com/inward/record.url?scp=0028578107&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028578107&partnerID=8YFLogxK

U2 - 10.1006/geno.1994.1633

DO - 10.1006/geno.1994.1633

M3 - Article

VL - 24

SP - 383

EP - 387

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 2

ER -