Molecular cloning and expression analysis of GFRα-3, a novel cDNA related to GDNFRα and NTNRα

Satoshi Nomoto, Sachiko Ito, Li Xia Yang, Kazutoshi Kiuchi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Glial-cell-line-derived neurotrophic factor (GDNF) and neurturin (NTN) are structurally related to TGF-β and are survival factors for sympathetic, sensory, and central nervous system neurons. GDNF transmits its signal primarily through a receptor complex containing the receptor tyrosine kinase Ret and a glycosyl-phosphatidylinositol (GPI)-linked receptor, GDNFRα. NTN utilizes a receptor complex system that consists of Ret and another GPI-linked receptor, NTNRα. We have identified a mouse cDNA, termed GFRα-3, that encodes a putative GPI-linked receptor. At the protein level, mouse GFRα-3 is 35% identical to mouse GDNFRα and 36% identical to mouse NTNRα. Northern blot analysis showed that GFRα-3 is expressed in fetal mouse heart, brain, lung, and kidney and adult heart. These results indicate that the tissue distribution of GFRα-3 mRNA is different from that of GDNFRα or NTNRα mRNA, and suggest that GFRα-3 may function in differentiation of embryonic cells expressing its mRNA.

Original languageEnglish
Pages (from-to)849-853
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume244
Issue number3
DOIs
Publication statusPublished - Mar 27 1998
Externally publishedYes

Fingerprint

Glycosylphosphatidylinositols
Cloning
Neurturin
Molecular Cloning
Glial Cell Line-Derived Neurotrophic Factor
Complementary DNA
Messenger RNA
Receptor Protein-Tyrosine Kinases
Neurology
Neurons
Large scale systems
Brain
Fetal Heart
Cells
Tissue
Sympathetic Nervous System
Tissue Distribution
Northern Blotting
Cell Differentiation
Central Nervous System

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Molecular cloning and expression analysis of GFRα-3, a novel cDNA related to GDNFRα and NTNRα. / Nomoto, Satoshi; Ito, Sachiko; Yang, Li Xia; Kiuchi, Kazutoshi.

In: Biochemical and Biophysical Research Communications, Vol. 244, No. 3, 27.03.1998, p. 849-853.

Research output: Contribution to journalArticle

Nomoto, Satoshi ; Ito, Sachiko ; Yang, Li Xia ; Kiuchi, Kazutoshi. / Molecular cloning and expression analysis of GFRα-3, a novel cDNA related to GDNFRα and NTNRα. In: Biochemical and Biophysical Research Communications. 1998 ; Vol. 244, No. 3. pp. 849-853.
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