Molecular cloning and expression of a 9-cis-epoxy carotenoid dioxygenase gene (NCED) and its relationship to dormancy in Lilium longiflorum and L. formosanum

Narges Mojtahedi, Jun Ichiro Masuda, Yukio Ozaki, Nguyen Thi Lam Hai, Kiyohide Kojima, Fumitaka Chino, Michikazu Hiramatsu, Yuki Mizunoe, Hiroshi Okubo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Individuals in northern populations of Lilium longiflorum in the Ryukyu Archipelago between Japan and Taiwan exhibit deep dormancy which is induced by high temperatures in the Summer, while plants of southern ecotypes and L. formosanum show no dormancy. In higher plants, 9-cis-epoxycarotenoid dioxygenase (NCED) is the critical enzyme for the regulation of abscisic acid (ABA) biosynthesis. The relationships between levels of expression of the LlNCED gene (GenBank Accession No. KC357734) and endogenous ABA levels were examined in 1-year-old seedlings of an L. formosanum population domesticated in Fukuoka (FFU) and in two L. longiflorum populations native to Pitouchiao (LPI), Taiwan, or Kikai Jima (LKI), Japan. The deduced amino acid sequences of the NCED proteins shared high identity with NCED proteins in other monocotyledons, and were closely related to enzymes in other members of the class Liliopsida. A high correlation was observed between levels of LlNCED gene expression and ABA concentration. Seedlings from LKI had the highest level of expression of LlNCED in August, while seedlings from FFU had the lowest level. Higher expression of the LlNCED gene in mid-Summer in the dormant population was related to higher endogenous concentrations of ABA. These results provide evidence that the induction of deep dormancy was initiated by the accumulation of ABA in dormant types of L. longiflorum, which was induced by high Summer temperatures.

Original languageEnglish
Pages (from-to)121-126
Number of pages6
JournalJournal of Horticultural Science and Biotechnology
Volume90
Issue number2
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

9-cis-epoxy-carotenoid dioxygenase
Lilium formosanum
Lilium
Lilium longiflorum
Abscisic Acid
Molecular Cloning
epoxides
dormancy
abscisic acid
molecular cloning
carotenoids
Seedlings
Genes
Liliopsida
Taiwan
Gene Expression
genes
gene expression
Population
seedlings

All Science Journal Classification (ASJC) codes

  • Genetics
  • Horticulture

Cite this

Molecular cloning and expression of a 9-cis-epoxy carotenoid dioxygenase gene (NCED) and its relationship to dormancy in Lilium longiflorum and L. formosanum. / Mojtahedi, Narges; Masuda, Jun Ichiro; Ozaki, Yukio; Hai, Nguyen Thi Lam; Kojima, Kiyohide; Chino, Fumitaka; Hiramatsu, Michikazu; Mizunoe, Yuki; Okubo, Hiroshi.

In: Journal of Horticultural Science and Biotechnology, Vol. 90, No. 2, 01.01.2015, p. 121-126.

Research output: Contribution to journalArticle

@article{c235a63fc5b74bc1886830af2e121d32,
title = "Molecular cloning and expression of a 9-cis-epoxy carotenoid dioxygenase gene (NCED) and its relationship to dormancy in Lilium longiflorum and L. formosanum",
abstract = "Individuals in northern populations of Lilium longiflorum in the Ryukyu Archipelago between Japan and Taiwan exhibit deep dormancy which is induced by high temperatures in the Summer, while plants of southern ecotypes and L. formosanum show no dormancy. In higher plants, 9-cis-epoxycarotenoid dioxygenase (NCED) is the critical enzyme for the regulation of abscisic acid (ABA) biosynthesis. The relationships between levels of expression of the LlNCED gene (GenBank Accession No. KC357734) and endogenous ABA levels were examined in 1-year-old seedlings of an L. formosanum population domesticated in Fukuoka (FFU) and in two L. longiflorum populations native to Pitouchiao (LPI), Taiwan, or Kikai Jima (LKI), Japan. The deduced amino acid sequences of the NCED proteins shared high identity with NCED proteins in other monocotyledons, and were closely related to enzymes in other members of the class Liliopsida. A high correlation was observed between levels of LlNCED gene expression and ABA concentration. Seedlings from LKI had the highest level of expression of LlNCED in August, while seedlings from FFU had the lowest level. Higher expression of the LlNCED gene in mid-Summer in the dormant population was related to higher endogenous concentrations of ABA. These results provide evidence that the induction of deep dormancy was initiated by the accumulation of ABA in dormant types of L. longiflorum, which was induced by high Summer temperatures.",
author = "Narges Mojtahedi and Masuda, {Jun Ichiro} and Yukio Ozaki and Hai, {Nguyen Thi Lam} and Kiyohide Kojima and Fumitaka Chino and Michikazu Hiramatsu and Yuki Mizunoe and Hiroshi Okubo",
year = "2015",
month = "1",
day = "1",
doi = "10.1080/14620316.2015.11513162",
language = "English",
volume = "90",
pages = "121--126",
journal = "Journal of Horticultural Science and Biotechnology",
issn = "1462-0316",
publisher = "Headley Brothers Ltd",
number = "2",

}

TY - JOUR

T1 - Molecular cloning and expression of a 9-cis-epoxy carotenoid dioxygenase gene (NCED) and its relationship to dormancy in Lilium longiflorum and L. formosanum

AU - Mojtahedi, Narges

AU - Masuda, Jun Ichiro

AU - Ozaki, Yukio

AU - Hai, Nguyen Thi Lam

AU - Kojima, Kiyohide

AU - Chino, Fumitaka

AU - Hiramatsu, Michikazu

AU - Mizunoe, Yuki

AU - Okubo, Hiroshi

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Individuals in northern populations of Lilium longiflorum in the Ryukyu Archipelago between Japan and Taiwan exhibit deep dormancy which is induced by high temperatures in the Summer, while plants of southern ecotypes and L. formosanum show no dormancy. In higher plants, 9-cis-epoxycarotenoid dioxygenase (NCED) is the critical enzyme for the regulation of abscisic acid (ABA) biosynthesis. The relationships between levels of expression of the LlNCED gene (GenBank Accession No. KC357734) and endogenous ABA levels were examined in 1-year-old seedlings of an L. formosanum population domesticated in Fukuoka (FFU) and in two L. longiflorum populations native to Pitouchiao (LPI), Taiwan, or Kikai Jima (LKI), Japan. The deduced amino acid sequences of the NCED proteins shared high identity with NCED proteins in other monocotyledons, and were closely related to enzymes in other members of the class Liliopsida. A high correlation was observed between levels of LlNCED gene expression and ABA concentration. Seedlings from LKI had the highest level of expression of LlNCED in August, while seedlings from FFU had the lowest level. Higher expression of the LlNCED gene in mid-Summer in the dormant population was related to higher endogenous concentrations of ABA. These results provide evidence that the induction of deep dormancy was initiated by the accumulation of ABA in dormant types of L. longiflorum, which was induced by high Summer temperatures.

AB - Individuals in northern populations of Lilium longiflorum in the Ryukyu Archipelago between Japan and Taiwan exhibit deep dormancy which is induced by high temperatures in the Summer, while plants of southern ecotypes and L. formosanum show no dormancy. In higher plants, 9-cis-epoxycarotenoid dioxygenase (NCED) is the critical enzyme for the regulation of abscisic acid (ABA) biosynthesis. The relationships between levels of expression of the LlNCED gene (GenBank Accession No. KC357734) and endogenous ABA levels were examined in 1-year-old seedlings of an L. formosanum population domesticated in Fukuoka (FFU) and in two L. longiflorum populations native to Pitouchiao (LPI), Taiwan, or Kikai Jima (LKI), Japan. The deduced amino acid sequences of the NCED proteins shared high identity with NCED proteins in other monocotyledons, and were closely related to enzymes in other members of the class Liliopsida. A high correlation was observed between levels of LlNCED gene expression and ABA concentration. Seedlings from LKI had the highest level of expression of LlNCED in August, while seedlings from FFU had the lowest level. Higher expression of the LlNCED gene in mid-Summer in the dormant population was related to higher endogenous concentrations of ABA. These results provide evidence that the induction of deep dormancy was initiated by the accumulation of ABA in dormant types of L. longiflorum, which was induced by high Summer temperatures.

UR - http://www.scopus.com/inward/record.url?scp=84941758680&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84941758680&partnerID=8YFLogxK

U2 - 10.1080/14620316.2015.11513162

DO - 10.1080/14620316.2015.11513162

M3 - Article

VL - 90

SP - 121

EP - 126

JO - Journal of Horticultural Science and Biotechnology

JF - Journal of Horticultural Science and Biotechnology

SN - 1462-0316

IS - 2

ER -