Molecular cloning and expression of a novel klotho-related protein

Kensei Yahata, Kiyoshi Mori, Hiroshi Arai, Susumu Koide, Yoshihiro Ogawa, Masashi Mukoyama, Akira Sugawara, Shoichi Ozaki, Issei Tanaka, Yo Ichi Nabeshima, Kazuwa Nakao

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Klotho protein is a novel β-glucosidase-like protein produced predominantly in the kidney. The klotho mouse, which genetically lacks klotho gene expression, manifests various systemic phenotypes resembling aging. In the present study we succeeded in isolating a novel human protein structurally related to klotho protein. The protein possesses one β-glucosidase-like domain and is 42% identical with klotho protein at the amino acid level. Unlike klotho protein, it possesses neither a signal sequence nor a transmembrane domain, suggesting that it is a cytosolic protein, and thus was termed cytosolic β-glucosidase-like protein-1 (cBGL1). By Northern blot analysis cBGL1 mRNA was expressed most abundantly in the liver, followed by the small intestine, colon, spleen, and kidney. When klotho and cBGL1 gene expression was examined in renal cell carcinoma tissues, both klotho and cBGL1 mRNA levels in tumors were lower than those in nontumor regions, suggesting that renal epithelial cells may lose klotho and cBGL1 gene expression during the course of malignant transformation. In conclusion, we describe the primary structure and gene expression of a novel protein related to klotho protein.

Original languageEnglish
Pages (from-to)389-394
Number of pages6
JournalJournal of Molecular Medicine
Volume78
Issue number7
DOIs
Publication statusPublished - Jan 1 2000

Fingerprint

Molecular Cloning
Glucosidases
Gene Expression
Proteins
Kidney
Messenger RNA
Protein Sorting Signals
Renal Cell Carcinoma
Northern Blotting
Small Intestine
klotho protein
Colon
Spleen
Epithelial Cells
Phenotype
Amino Acids
Liver
Neoplasms

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Drug Discovery
  • Genetics(clinical)

Cite this

Yahata, K., Mori, K., Arai, H., Koide, S., Ogawa, Y., Mukoyama, M., ... Nakao, K. (2000). Molecular cloning and expression of a novel klotho-related protein. Journal of Molecular Medicine, 78(7), 389-394. https://doi.org/10.1007/s001090000131

Molecular cloning and expression of a novel klotho-related protein. / Yahata, Kensei; Mori, Kiyoshi; Arai, Hiroshi; Koide, Susumu; Ogawa, Yoshihiro; Mukoyama, Masashi; Sugawara, Akira; Ozaki, Shoichi; Tanaka, Issei; Nabeshima, Yo Ichi; Nakao, Kazuwa.

In: Journal of Molecular Medicine, Vol. 78, No. 7, 01.01.2000, p. 389-394.

Research output: Contribution to journalArticle

Yahata, K, Mori, K, Arai, H, Koide, S, Ogawa, Y, Mukoyama, M, Sugawara, A, Ozaki, S, Tanaka, I, Nabeshima, YI & Nakao, K 2000, 'Molecular cloning and expression of a novel klotho-related protein', Journal of Molecular Medicine, vol. 78, no. 7, pp. 389-394. https://doi.org/10.1007/s001090000131
Yahata, Kensei ; Mori, Kiyoshi ; Arai, Hiroshi ; Koide, Susumu ; Ogawa, Yoshihiro ; Mukoyama, Masashi ; Sugawara, Akira ; Ozaki, Shoichi ; Tanaka, Issei ; Nabeshima, Yo Ichi ; Nakao, Kazuwa. / Molecular cloning and expression of a novel klotho-related protein. In: Journal of Molecular Medicine. 2000 ; Vol. 78, No. 7. pp. 389-394.
@article{d7341e80961e484eac47581a465cb352,
title = "Molecular cloning and expression of a novel klotho-related protein",
abstract = "Klotho protein is a novel β-glucosidase-like protein produced predominantly in the kidney. The klotho mouse, which genetically lacks klotho gene expression, manifests various systemic phenotypes resembling aging. In the present study we succeeded in isolating a novel human protein structurally related to klotho protein. The protein possesses one β-glucosidase-like domain and is 42{\%} identical with klotho protein at the amino acid level. Unlike klotho protein, it possesses neither a signal sequence nor a transmembrane domain, suggesting that it is a cytosolic protein, and thus was termed cytosolic β-glucosidase-like protein-1 (cBGL1). By Northern blot analysis cBGL1 mRNA was expressed most abundantly in the liver, followed by the small intestine, colon, spleen, and kidney. When klotho and cBGL1 gene expression was examined in renal cell carcinoma tissues, both klotho and cBGL1 mRNA levels in tumors were lower than those in nontumor regions, suggesting that renal epithelial cells may lose klotho and cBGL1 gene expression during the course of malignant transformation. In conclusion, we describe the primary structure and gene expression of a novel protein related to klotho protein.",
author = "Kensei Yahata and Kiyoshi Mori and Hiroshi Arai and Susumu Koide and Yoshihiro Ogawa and Masashi Mukoyama and Akira Sugawara and Shoichi Ozaki and Issei Tanaka and Nabeshima, {Yo Ichi} and Kazuwa Nakao",
year = "2000",
month = "1",
day = "1",
doi = "10.1007/s001090000131",
language = "English",
volume = "78",
pages = "389--394",
journal = "Clinical Investigator",
issn = "0946-2716",
publisher = "Springer Verlag",
number = "7",

}

TY - JOUR

T1 - Molecular cloning and expression of a novel klotho-related protein

AU - Yahata, Kensei

AU - Mori, Kiyoshi

AU - Arai, Hiroshi

AU - Koide, Susumu

AU - Ogawa, Yoshihiro

AU - Mukoyama, Masashi

AU - Sugawara, Akira

AU - Ozaki, Shoichi

AU - Tanaka, Issei

AU - Nabeshima, Yo Ichi

AU - Nakao, Kazuwa

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Klotho protein is a novel β-glucosidase-like protein produced predominantly in the kidney. The klotho mouse, which genetically lacks klotho gene expression, manifests various systemic phenotypes resembling aging. In the present study we succeeded in isolating a novel human protein structurally related to klotho protein. The protein possesses one β-glucosidase-like domain and is 42% identical with klotho protein at the amino acid level. Unlike klotho protein, it possesses neither a signal sequence nor a transmembrane domain, suggesting that it is a cytosolic protein, and thus was termed cytosolic β-glucosidase-like protein-1 (cBGL1). By Northern blot analysis cBGL1 mRNA was expressed most abundantly in the liver, followed by the small intestine, colon, spleen, and kidney. When klotho and cBGL1 gene expression was examined in renal cell carcinoma tissues, both klotho and cBGL1 mRNA levels in tumors were lower than those in nontumor regions, suggesting that renal epithelial cells may lose klotho and cBGL1 gene expression during the course of malignant transformation. In conclusion, we describe the primary structure and gene expression of a novel protein related to klotho protein.

AB - Klotho protein is a novel β-glucosidase-like protein produced predominantly in the kidney. The klotho mouse, which genetically lacks klotho gene expression, manifests various systemic phenotypes resembling aging. In the present study we succeeded in isolating a novel human protein structurally related to klotho protein. The protein possesses one β-glucosidase-like domain and is 42% identical with klotho protein at the amino acid level. Unlike klotho protein, it possesses neither a signal sequence nor a transmembrane domain, suggesting that it is a cytosolic protein, and thus was termed cytosolic β-glucosidase-like protein-1 (cBGL1). By Northern blot analysis cBGL1 mRNA was expressed most abundantly in the liver, followed by the small intestine, colon, spleen, and kidney. When klotho and cBGL1 gene expression was examined in renal cell carcinoma tissues, both klotho and cBGL1 mRNA levels in tumors were lower than those in nontumor regions, suggesting that renal epithelial cells may lose klotho and cBGL1 gene expression during the course of malignant transformation. In conclusion, we describe the primary structure and gene expression of a novel protein related to klotho protein.

UR - http://www.scopus.com/inward/record.url?scp=0033827077&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033827077&partnerID=8YFLogxK

U2 - 10.1007/s001090000131

DO - 10.1007/s001090000131

M3 - Article

C2 - 11043382

AN - SCOPUS:0033827077

VL - 78

SP - 389

EP - 394

JO - Clinical Investigator

JF - Clinical Investigator

SN - 0946-2716

IS - 7

ER -