Molecular cloning and expression of human hepatocyte growth factor

Toshikazu Nakamura, Tsutomu Nishizawa, Mitchio Hagiya, Tatsuya Seki, Manabu Shimonishi, Atsushi Sugimura, Kosuke Tashiro, Shin Shimizu

Research output: Contribution to journalArticlepeer-review

1915 Citations (Scopus)

Abstract

Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchyma! hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. The partial purification and characterization of HGF have been reported1-4. We have demonstrated that pure HGF from rat platelets is a new growth factor5, 6 effective at concentrations as low as 1 ng mP-1.The effects of HGF and epidermal growth factor (EGF) are additive. The activity of HGF is not species-specific, although it does not stimulate growth in Swiss 3T3 fibroblasts5. HGF has a relative molecular mass (Mr) of 82,000 and is a heterodimer composed of a large a-subunit of Mr 69,000 and a small β -subunit of Mr34,000 (ref. 6). Here we report the amino-acid sequence of human HGF determined by complementary DNA cloning and the expression of biologically active human HGF from COS-1 cells transfected with cloned cDNA. The nucleotide sequence of the human HGF cDNA reveals that both α- andβ-chains are contained in a single open reading frame coding for a pre-pro precursor protein of 728 amino acids.

Original languageEnglish
Pages (from-to)440-443
Number of pages4
JournalNature
Volume342
Issue number6248
DOIs
Publication statusPublished - 1989

All Science Journal Classification (ASJC) codes

  • General

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