Abstract
We isolated two types of hamster cyclin-dependent kinase 2 (cdk2) cDNAs from BHK21 cells derived from Golden hamsters. One type of cdk2 (cdk2hm) encodes the 32 kDa protein consisting of 298 predicted amino acids and shows strong homology to the cdk2 cDNAs of humans and Xenopus. The other cdk2 (cdk2Lhm) encodes the 38 kDa protein containing the insertion of 48 amino acids in the cdk2hm protein. Immunoblotting analysis suggested that these two types of cdk2 protein exist in mammalian cells. The cdk2hm has the activity of protein kinase, while the cdk2Lhm does not, however, both bind with cyclin E.
| Original language | English |
|---|---|
| Pages (from-to) | 1524-1529 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 197 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Dec 30 1993 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
Molecular cloning and identification of two types of hamster cyclin-dependent kinases : CDK2 and CDK2L. / Noguchi, Eishi; Sekiguchi, Takeshi; Yamashita, Katsumi; Nishimoto, Takeharu.
In: Biochemical and Biophysical Research Communications, Vol. 197, No. 3, 30.12.1993, p. 1524-1529.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular cloning and identification of two types of hamster cyclin-dependent kinases
T2 - CDK2 and CDK2L
AU - Noguchi, Eishi
AU - Sekiguchi, Takeshi
AU - Yamashita, Katsumi
AU - Nishimoto, Takeharu
PY - 1993/12/30
Y1 - 1993/12/30
N2 - We isolated two types of hamster cyclin-dependent kinase 2 (cdk2) cDNAs from BHK21 cells derived from Golden hamsters. One type of cdk2 (cdk2hm) encodes the 32 kDa protein consisting of 298 predicted amino acids and shows strong homology to the cdk2 cDNAs of humans and Xenopus. The other cdk2 (cdk2Lhm) encodes the 38 kDa protein containing the insertion of 48 amino acids in the cdk2hm protein. Immunoblotting analysis suggested that these two types of cdk2 protein exist in mammalian cells. The cdk2hm has the activity of protein kinase, while the cdk2Lhm does not, however, both bind with cyclin E.
AB - We isolated two types of hamster cyclin-dependent kinase 2 (cdk2) cDNAs from BHK21 cells derived from Golden hamsters. One type of cdk2 (cdk2hm) encodes the 32 kDa protein consisting of 298 predicted amino acids and shows strong homology to the cdk2 cDNAs of humans and Xenopus. The other cdk2 (cdk2Lhm) encodes the 38 kDa protein containing the insertion of 48 amino acids in the cdk2hm protein. Immunoblotting analysis suggested that these two types of cdk2 protein exist in mammalian cells. The cdk2hm has the activity of protein kinase, while the cdk2Lhm does not, however, both bind with cyclin E.
UR - http://www.scopus.com/inward/record.url?scp=0027751631&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027751631&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1993.2650
DO - 10.1006/bbrc.1993.2650
M3 - Article
C2 - 8280171
AN - SCOPUS:0027751631
VL - 197
SP - 1524
EP - 1529
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -