Molecular cloning and localization of a CEACAM2 isoform, CEACAM2-L, expressed in spermatids in mouse testis

Elsaid Salaheldeen, Hitoshi Kurio, Ali Howida, Hiroshi Iida

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Carcinoembryonic antigen (CEA) family, a subgroup of the immunoglobulin (Ig) superfamily, is divided into two sub-families: the CEA-related cell adhesion molecules (CEACAM) and the pregnancy-specific glycoproteins. The isoform CEACAM2 is expressed in mouse testis; in this study, we identified a novel isoform of Ceacam2, Ceacam2-Long (Ceacam2-L). CEACAM2-L is different from CEACAM2 in that it has much longer cytoplasmic tail region. Ceacam2-L starts to appear faintly in mouse testis after 3 weeks of postnatal development, and its expression level increased after 5 weeks. Immunoblot analysis confirmed the expression of CEACAM2-L in the seminiferous epithelium of mouse testis. Immunohistochemical data showed that CEACAM2-L was not observed on spermatogonia, spermatocytes, round spermatids, or Sertoli cells, but was seen at the plasma membrane of elongating spermatids in contact with extended cytoplasmic processes of Sertoli cells. CEACAM2-L was not detected at the head region of elongating spermatids, where the apical ectoplasmic specialization is constructed. These data suggest that CEACAM2-L might be a novel adhesion molecule contributing to cell-to-cell adhesion between elongating spermatids and Sertoli cells within the seminiferous epithelium.

Original languageEnglish
Pages (from-to)843-852
Number of pages10
JournalMolecular Reproduction and Development
Volume79
Issue number12
DOIs
Publication statusPublished - Dec 2012

Fingerprint

Spermatids
Molecular Cloning
Testis
Protein Isoforms
Sertoli Cells
Seminiferous Epithelium
Carcinoembryonic Antigen
Spermatogonia
Spermatocytes
Cell Adhesion Molecules
CD66 antigens
Cell Adhesion
Tail
Immunoglobulins
Glycoproteins
Head
Cell Membrane
Pregnancy

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Molecular cloning and localization of a CEACAM2 isoform, CEACAM2-L, expressed in spermatids in mouse testis. / Salaheldeen, Elsaid; Kurio, Hitoshi; Howida, Ali; Iida, Hiroshi.

In: Molecular Reproduction and Development, Vol. 79, No. 12, 12.2012, p. 843-852.

Research output: Contribution to journalArticle

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